Published in:
01-06-2019 | Original Research
Expression Level of miR-34a in Tumor Tissue from Patients with Esophageal Squamous Cell Carcinoma
Authors:
Milad Asadi, Dariush Shanehbandi, Hanieh Mohammadpour, Shahriyar Hashemzadeh, Bita Sepehri
Published in:
Journal of Gastrointestinal Cancer
|
Issue 2/2019
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Abstract
Background
miR-34a has been shown to be involved in P53 regulation. In this study, we aimed to evaluate the expression level of miR-34a in esophageal cancer and compare it with that of the normal marginal tissues.
Methods
Tumor and marginal tissues were obtained from 50 patients with esophageal cancer. After RNA extraction, expression level of miR-34a was determined using SYBR green master mix and real-time quantitative PCR.
Results
It was observed that there was a downregulation of miR-34a in tumoral tissue of esophageal patients in comparison to normal marginal tissues. Moreover, the expression level of miR-34a was correlated with clinicopathological specifications of the patients.
Conclusions
miR-34a may be involved in the pathogenesis and development of esophageal cancer and have the potential to be used as a diagnostic and therapeutic marker in esophageal cancer.