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Immunologic Research
Published in: Immunologic Research 4/2017

01-08-2017 | Original Article

Thymus vulgaris L. and thymol assist murine macrophages (RAW 264.7) in the control of in vitro infections by Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans

Authors: Jonatas Rafael de Oliveira, Leandro Wagner Figueira, Fábia Lugli Sper, Vanessa Marques Meccatti, Samira Esteves Afonso Camargo, Luciane Dias de Oliveira

Published in: Immunologic Research | Issue 4/2017

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Abstract

Microorganisms are capable to combat defense cells by means of strategies that contribute to their stabilization and proliferation in invaded tissues. Frequently antimicrobial-resistant strains appear; therefore, alternative methods to control them must be investigated, for example, the use of plant products. The capacity of the thyme extract (Thymus vulgaris L.) and phytocompound thymol in the control of in vitro infections by Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans in murine macrophages (RAW 264.7) was evaluated. Minimal inhibitory concentrations (MIC) of the plant products were used. The effect of these MIC were analyzed in the assays of phagocytosis and immunoregulation by analysis of the production of cytokines (IL-1β, TNF-α, and IL-10) and nitric oxide (NO). The plant products effectively assisted the macrophages in the phagocytosis of microorganisms, presenting significant reductions of S. aureus and P. aeruginosa. The macrophages also regulated the production of inflammatory mediators in the infections by S. aureus, P. aeruginosa, and C. albicans. In addition, thyme provided a satisfactory effect in response to the bacterial infections, regarding generation of NO. Thus, the effectiveness of the thyme and thymol to control in vitro infections by S. aureus, P. aeruginosa, and C. albicans was observed.

Highlights

  • Phagocytosis of S. aureus by RAW 264.7 was enhanced with thymol
  • Thyme enhanced the phagocytosis of P. aeruginosa by RAW 264.7
  • Plant products provided immunoregulation of inflammatory cytokines
  • Production of nitric oxide was improved with the treatments in bacterial infections
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Metadata
Title
Thymus vulgaris L. and thymol assist murine macrophages (RAW 264.7) in the control of in vitro infections by Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans
Authors
Jonatas Rafael de Oliveira
Leandro Wagner Figueira
Fábia Lugli Sper
Vanessa Marques Meccatti
Samira Esteves Afonso Camargo
Luciane Dias de Oliveira
Publication date
01-08-2017
Publisher
Springer US
Published in
Immunologic Research / Issue 4/2017
Print ISSN: 0257-277X
Electronic ISSN: 1559-0755
DOI
https://doi.org/10.1007/s12026-017-8933-z

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