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Endocrine
Published in: Endocrine 2/2016

01-08-2016 | Original Article

MCM2 mediates progesterone-induced endometrial stromal cell proliferation and differentiation in mice

Authors: Shuangbo Kong, Xue Han, Tongtong Cui, Chan Zhou, Yufei Jiang, Hangxiao Zhang, Bingyan Wang, Haibin Wang, Shuang Zhang

Published in: Endocrine | Issue 2/2016

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Abstract

Uterine decidualization characterized by stromal cell proliferation and differentiation is critical to the establishment of pregnancy in many species. Progesterone is a key factor in regulating endometrial cell decidualization, however, the molecular basis involved in mediating the effects of progesterone during decidualization remains largely unknown. We report here that the DNA replication licensing factor MCM2, one of the conserved set of six-related proteins (MCM complex: MCM2–7) essential for eukaryotic DNA replication, is dynamically expressed in both proliferative and differentiated stromal cells during mouse periimplantation uterus. Applying PR-knockout mouse model and pharmacological strategy, we further found that the expression of Mcm2 is induced by progesterone action in the mouse uterine stroma. Employing a primary cell culture system, we further demonstrated that siRNA-mediated silencing of MCM2 arrests the cell cycle at G1–S transition during stromal cell proliferation. Moreover, the downregulation of Mcm2 could also compromise stromal cell differentiation. Collectively, our studies uncovered the role of a unique DNA replication licensing molecule MCM2 in mediating Progesterone-induced stromal cell decidualization in mouse uterus.
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Metadata
Title
MCM2 mediates progesterone-induced endometrial stromal cell proliferation and differentiation in mice
Authors
Shuangbo Kong
Xue Han
Tongtong Cui
Chan Zhou
Yufei Jiang
Hangxiao Zhang
Bingyan Wang
Haibin Wang
Shuang Zhang
Publication date
01-08-2016
Publisher
Springer US
Published in
Endocrine / Issue 2/2016
Print ISSN: 1355-008X
Electronic ISSN: 1559-0100
DOI
https://doi.org/10.1007/s12020-016-0894-9

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