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Endocrine
Published in: Endocrine 3/2015

01-04-2015 | Original Article

Asymmetric steroidogenic response by the ovaries to the vasoactive intestinal peptide

Authors: Gabriela Rosas, María Isabel Ramírez, Rosa Linares, Angélica Trujillo, Roberto Domínguez, Leticia Morales-Ledesma

Published in: Endocrine | Issue 3/2015

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Abstract

In vitro the vasoactive intestinal peptide (VIP) stimulates progesterone, androgens, and estradiol secretion, and the effects are time-dependent. The present study analyzed the acute (1 h) and sub-acute (24 h) effects of unilateral injection of VIP into the ovarian bursa on each day of the estrous cycle on progesterone, testosterone, and estradiol serum levels. Cyclic 60-day-old virgin female rats on diestrus-1, diestrus-2, proestrus, or estrus were injected with saline or VIP 10−6 M into the left or right ovarian bursa. One hour after saline injection on each day of estrus cycle, progesterone levels were higher than in control animals. The acute effects of saline solution on testosterone and estradiol levels were asymmetric and varied during the estrous cycle. In comparison with saline groups, the effects of VIPergic stimulation on progesterone, testosterone, and estradiol serum levels depend on the time elapsed between treatment and autopsy and vary during the estrous cycle. An acute asymmetric response from the ovaries to the VIP was observed at diestrus-1, diestrus-2, and proestrus on progesterone and estradiol levels. The asymmetries on testosterone levels were observed at diestrus-1, diestrus-2, and estrus days. The present results suggest that in the cyclic rat, each ovary has different sensitivities to VIPergic stimulation which depends on the endocrine status of the animal.
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Metadata
Title
Asymmetric steroidogenic response by the ovaries to the vasoactive intestinal peptide
Authors
Gabriela Rosas
María Isabel Ramírez
Rosa Linares
Angélica Trujillo
Roberto Domínguez
Leticia Morales-Ledesma
Publication date
01-04-2015
Publisher
Springer US
Published in
Endocrine / Issue 3/2015
Print ISSN: 1355-008X
Electronic ISSN: 1559-0100
DOI
https://doi.org/10.1007/s12020-014-0449-x

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