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NeuroMolecular Medicine
Published in: NeuroMolecular Medicine 4/2017

01-12-2017 | Original Paper

Purine Biosynthesis Enzymes in Hippocampal Neurons

Authors: Julie Williamson, Ronald S. Petralia, Ya-Xian Wang, Mark P. Mattson, Pamela J. Yao

Published in: NeuroMolecular Medicine | Issue 4/2017

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Abstract

Despite reports implicating disrupted purine metabolism in causing a wide spectrum of neurological defects, the mechanistic details of purine biosynthesis in neurons are largely unknown. As an initial step in filling that gap, we examined the expression and subcellular distribution of three purine biosynthesis enzymes (PFAS, PAICS and ATIC) in rat hippocampal neurons. Using immunoblotting and high-resolution light and electron microscopic analysis, we find that all three enzymes are broadly distributed in hippocampal neurons with pools of these enzymes associated with mitochondria. These findings suggest a potential link between purine metabolism and mitochondrial function in neurons and provide an impetus for further studies.
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Metadata
Title
Purine Biosynthesis Enzymes in Hippocampal Neurons
Authors
Julie Williamson
Ronald S. Petralia
Ya-Xian Wang
Mark P. Mattson
Pamela J. Yao
Publication date
01-12-2017
Publisher
Springer US
Published in
NeuroMolecular Medicine / Issue 4/2017
Print ISSN: 1535-1084
Electronic ISSN: 1559-1174
DOI
https://doi.org/10.1007/s12017-017-8466-6

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