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Cardiovascular Toxicology
Published in: Cardiovascular Toxicology 3/2017

01-07-2017

H2S Prevents Cyclosporine A-Induced Vasomotor Alteration in Rats

Authors: Na-na Ping, Yan-ni Mi, Dong-zheng Liu, Sai Zhang, Jing-guo Chen, Yong-xiao Cao

Published in: Cardiovascular Toxicology | Issue 3/2017

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Abstract

Cyclosporine A (CsA) induces hypertension after transplantation. Hydrogen sulfide (H2S) was found to have hypotensive/vasoprotective effects in the cardiovascular system. The present study aims to investigate the role of H2S on CsA-induced vascular function disorder in rats. Rats were subcutaneously injected with CsA 25 mg/kg for 21 days. Blood pressure was measured by the tail-cuff method. Vasomotion was determined using a sensitive myograph. Western blotting and immunohistochemistry were used to quantify the protein expression of endothelin type A (ETA) receptor and essential MAPK pathway molecules. Vascular superoxide anion production and serum contents of malondialdehyde were determined. The results showed that sodium hydrosulfide (NaHS), a H2S donor, significantly attenuated the increase of blood pressure and contractile responses, and the upregulation of ETA receptor induced by CsA. In addition, NaHS could restore the CsA decreased acetylcholine-induced vasodilatation. Furthermore, NaHS blocked the CsA-induced elevation of reactive oxygen species level, extracellular signal-regulated kinase and p38 MAPK activities. In conclusion, H2S prevents CsA-induced vasomotor dysfunction. H2S attenuates CsA-induced ETA receptor upregulation, which may be associated with MAPK signal pathways. H2S ameliorates endothelial-dependent relaxation, which may be through antioxidant activity.
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Metadata
Title
H2S Prevents Cyclosporine A-Induced Vasomotor Alteration in Rats
Authors
Na-na Ping
Yan-ni Mi
Dong-zheng Liu
Sai Zhang
Jing-guo Chen
Yong-xiao Cao
Publication date
01-07-2017
Publisher
Springer US
Published in
Cardiovascular Toxicology / Issue 3/2017
Print ISSN: 1530-7905
Electronic ISSN: 1559-0259
DOI
https://doi.org/10.1007/s12012-016-9383-x

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