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Cardiovascular Toxicology
Published in: Cardiovascular Toxicology 3/2010

01-09-2010

Purine Bases Oxidation and Repair Following Permethrin Insecticide Treatment in Rat Heart Cells

Authors: M. S. Dhivya Vadhana, Cinzia Nasuti, Rosita Gabbianelli

Published in: Cardiovascular Toxicology | Issue 3/2010

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Abstract

Pollutants including insecticides have been recently reported to be a risk factor involved in various diseases. Permethrin, a member of the family of synthetic pyrethroids, is widely used as insecticide in agriculture and other domestic applications. To investigate possible cardiotoxicity, we had examined different concentrations of permethrin on the freshly isolated rat heart cells using the alkaline comet assay. A significant difference in % tail DNA between all concentrations of permethrin (5, 10, 20 μM) and vehicle (control) without enzymes and with Fpg-treated cells were measured. The results indicated that permethrin induced oxidative damage to purine bases in the heart cells. Pyrimidines oxidation was evaluated using Endonuclease III (Endo III), but the results did not reveal any significant changes. After permethrin exposure, cells were studied to evaluate their DNA repair capacity. A complete DNA repair at 10 and 20 μM was measured after 30 and 60 min of repair intervals. Significant change in plasma membrane fluidity at different depths of bilayer was measured following permethrin treatment. Membrane fluidity in the hydrophilic–hydrophobic region was reduced, while the hydrophobic inner resulted more fluid following permethrin treatment of heart cells. This work points to standardize conditions applicable to ex vivo cells following in vivo treatment in order to study the cardiotoxicity of insecticide.
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Metadata
Title
Purine Bases Oxidation and Repair Following Permethrin Insecticide Treatment in Rat Heart Cells
Authors
M. S. Dhivya Vadhana
Cinzia Nasuti
Rosita Gabbianelli
Publication date
01-09-2010
Publisher
Humana Press Inc
Published in
Cardiovascular Toxicology / Issue 3/2010
Print ISSN: 1530-7905
Electronic ISSN: 1559-0259
DOI
https://doi.org/10.1007/s12012-010-9079-6

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