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Clinical Orthopaedics and Related Research®

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01-12-2009 | Symposium: Tribute to Dr. Marshall Urist: Musculoskeletal Growth Factors

OP-1 Augments Glucocorticoid-inhibited Fracture Healing in a Rat Fracture Model

Authors: Robert S. Gilley, DVM, PhD, DACVS, Larry J. Wallace, DVM, MS, DACVS, Craig A. Bourgeault, BS, Louis S. Kidder, PhD, Joan E. Bechtold, PhD

Published in: Clinical Orthopaedics and Related Research® | Issue 12/2009

Abstract

Glucocorticoids inhibit bone remodeling and fracture healing. We sought to determine whether osteogenic protein 1 (OP-1) can overcome this inhibition in a closed fracture model in the rat. Time-released prednisolone or placebo pellets were implanted subcutaneously; closed femoral fractures were created 2 weeks later in rats. Fractures received sham, OP-1 and collagen, or collagen-only implants. Femurs were harvested at 3, 10, 21, 28, and 42 days postfracture. Fractures were examined radiographically for amount of hard callus; mechanically for torque and stiffness (also expressed as a percentage of the contralateral intact femur); and histomorphometrically for amount of cartilaginous and noncartilaginous soft callus, hard callus, and total callus. Glucocorticoid administration inhibited fracture healing. The application of a devitalized Type I collagen matrix mitigated the inhibitory effects of prednisolone on fracture healing However, further increases in indices of fracture healing were observed when OP-1 was added to the collagen matrix compared with collagen alone. OP-1 and collagen was more effective than collagen alone.

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Title: OP-1 Augments Glucocorticoid-inhibited Fracture Healing in a Rat Fracture Model
Authors: Robert S. Gilley, DVM, PhD, DACVS
Larry J. Wallace, DVM, MS, DACVS
Craig A. Bourgeault, BS
Louis S. Kidder, PhD
Joan E. Bechtold, PhD
Publication date: 01-12-2009
Publisher: Springer-Verlag
Published in: Clinical Orthopaedics and Related Research® / Issue 12/2009
Print ISSN: 0009-921X
Electronic ISSN: 1528-1132
DOI: https://doi.org/10.1007/s11999-009-0782-1

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OP-1 Augments Glucocorticoid-inhibited Fracture Healing in a Rat Fracture Model

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