Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Irish Journal of Medical Science (1971 -)
Published in: Irish Journal of Medical Science (1971 -) 4/2017

01-11-2017 | Original Article

CCK1 receptor is involved in the regulation of protein lysine acetylation in GBC-SD cells and gallbladder carcinoma

Authors: W. Wu, B. Ouyang, Z. Lu, H. Liu, Y. Tan, P. Cui

Published in: Irish Journal of Medical Science (1971 -) | Issue 4/2017

Login to get access

Abstract

Background

CCK1 (cholecystokinin receptor 1) and protein lysine acetylation were associated with several cancers, respectively. However, whether they are involved in the alternation of gallbladder carcinoma is unknown.

Aims

This study investigated the characteristics of CCK1 and protein lysine acetylation in GBC-SD cells and carcinoma of gallbladder.

Methods

The expression and localization of CCK1 were detected by western blot analysis and indirect immunofluorescence. GBC-SD cells were treated with CCK-8 and CCK-8+CCK1 inhibitor. The protein lysine acetylation from cells, as well as tissues of gallbladder carcinoma, was examined by western blotting.

Results

CCK1 receptor was expressed and localized in the GBC-SD cells. The synthetic octapeptide of CCK (CCK-8) could accelerate the lysine acetylation of a subset of proteins in dose-dependent manners in GBC-SD cells. Further investigation demonstrated that the specific inhibitor (CR1409) of CCK1 receptor could attenuate the CCK8-induced increase of protein lysine acetylation. In addition, we revealed that the rise of CCK1 receptor expression is associated with the increase of protein lysine acetylation in tissues from carcinoma of gallbladder.

Conclusions

CCK might regulate protein lysine acetylation via CCK1 receptor.
Literature
1.
2.
3.
4.
Vanhoutte PM, Humphrey PP, Spedding M (1996) X. International Union of Pharmacology recommendations for nomenclature of new receptor subtypes. Pharmacol Rev 48:1–2PubMed
5.
Honda T, Wada E, Battey JF, Wank SA (1993) Differential gene expression of CCK(A) and CCK(B) receptors in the rat brain. Mol Cell Neurosci 4:143–154CrossRefPubMed
6.
Wank SA (1995) Cholecystokinin receptors. Am J Phys 269:G628–G646
7.
Matsusue K, Takiguchi S, Takata Y, Funakoshi A, Miyasaka K et al (1999) Expression of cholecystokinin type A receptor gene correlates with DNA demethylation during postnatal development of rat pancreas. Biochem Biophys Res Commun 264:29–32CrossRefPubMed
8.
Hughes J, Boden P, Costall B, Domeney A, Kelly E et al (1990) Development of a class of selective cholecystokinin type B receptor antagonists having potent anxiolytic activity. Proc Natl Acad Sci U S A 87:6728–6732CrossRefPubMedPubMedCentral
9.
Miyasaka K, Kanai S, Ohta M, Kawanami T, Kono A et al (1994) Lack of satiety effect of cholecystokinin (CCK) in a new rat model not expressing the CCK-A receptor gene. Neurosci Lett 180:143–146CrossRefPubMed
10.
Miyasaka K, Kobayashi S, Ohta M, Kanai S, Yoshida Y et al (2002) Anxiety-related behaviors in cholecystokinin-A, B, and AB receptor gene knockout mice in the plus-maze. Neurosci Lett 335:115–118CrossRefPubMed
11.
Rehfeld JF (2016) Cholecystokinin expression in tumors: biogenetic and diagnostic implications. Future Oncol 12:2135–2147CrossRefPubMed
12.
Mandair KK, Towner P, Stamford IF, Morris JD, Harper E et al (1998) Cholecystokinin receptors in human pancreatic cancer cell lines. Eur J Cancer 34:1455–1459CrossRefPubMed
13.
Rai R, Chandra V, Tewari M, Kumar M, Shukla HS (2012) Cholecystokinin and gastrin receptors targeting in gastrointestinal cancer. Surg Oncol 21:281–292CrossRefPubMed
14.
Smith JP, Solomon TE (2014) Cholecystokinin and pancreatic cancer: the chicken or the egg? Am J Physiol Gastrointest Liver Physiol 306:G91–G101CrossRefPubMed
15.
Piehler JM, Crichlow RW (1978) Primary carcinoma of the gallbladder. Surg Gynecol Obstet 147:929–942PubMed
16.
Ho WL, Hsu WM, Huang MC, Kadomatsu K, Nakagawara A (2016) Protein glycosylation in cancers and its potential therapeutic applications in neuroblastoma. J Hematol Oncol 9:100CrossRefPubMedPubMedCentral
17.
Li NY, Weber CE, Mi Z, Wai PY, Cuevas BD et al (2013) Osteopontin up-regulates critical epithelial-mesenchymal transition transcription factors to induce an aggressive breast cancer phenotype. J Am Coll Surg 217:17–26 discussion 26 CrossRefPubMed
18.
Iyer NG, Chin SF, Ozdag H, Daigo Y, Hu DE et al (2004) p300 regulates p53-dependent apoptosis after DNA damage in colorectal cancer cells by modulation of PUMA/p21 levels. Proc Natl Acad Sci U S A 101:7386–7391CrossRefPubMedPubMedCentral
19.
Zhou Y, Ru Y, Shi H, Wang Y, Wu B et al (2015) Cholecystokinin receptors regulate sperm protein tyrosine phosphorylation via uptake of HCO3. Reproduction 150:257–268CrossRefPubMed
20.
Yu H, Diao H, Wang C, Lin Y, Yu F et al (2015) Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm). Mol Cell Proteomics 14:1009–1023CrossRefPubMedPubMedCentral
21.
Wank SA, Harkins R, Jensen RT, Shapira H, de Weerth A et al (1992) Purification, molecular cloning, and functional expression of the cholecystokinin receptor from rat pancreas. Proc Natl Acad Sci U S A 89:3125–3129CrossRefPubMedPubMedCentral
22.
Kulaksiz H, Arnold R, Goke B, Maronde E, Meyer M et al (2000) Expression and cell-specific localization of the cholecystokinin B/gastrin receptor in the human stomach. Cell Tissue Res 299:289–298CrossRefPubMed
23.
24.
Moonka R, Zhou W, Bell RH Jr (1999) Cholecystokinin-A receptor messenger RNA expression in human pancreatic cancer. J Gastrointest Surg 3:134–140CrossRefPubMed
25.
Srivastava A, Pandey SN, Dixit M, Choudhuri G, Mittal B (2008) Cholecystokinin receptor A gene polymorphism in gallstone disease and gallbladder cancer. J Gastroenterol Hepatol 23:970–975CrossRefPubMed
26.
Faridi MS, Jaiswal MS, Goel SK (2015) Expression of CCK receptors in carcinoma gallbladder and cholelithiasis: a pilot study. J Clin Diagn Res 9:PC04–PC07PubMedPubMedCentral
27.
Kazmi HR, Chandra A, Baghel K, Singh A, Nigam J et al (2014) Differential expression of cholecystokinin A receptor in gallbladder cancer in the young and elderly suggests two subsets of the same disease? Biomed Res Int 2014:625695CrossRefPubMedPubMedCentral
28.
Allfrey VG, Faulkner R, Mirsky AE (1964) Acetylation and methylation of histones and their possible role in the regulation of RNA synthesis. Proc Natl Acad Sci U S A 51:786–794CrossRefPubMedPubMedCentral
29.
30.
Gil J, Ramirez-Torres A, Encarnacion-Guevara S (2017) Lysine acetylation and cancer: A proteomics perspective. J Proteome 150:297–309CrossRef
31.
Di Gennaro E, Bruzzese F, Caraglia M, Abruzzese A, Budillon A (2004) Acetylation of proteins as novel target for antitumor therapy: review article. Amino Acids 26:435–441CrossRefPubMed
Metadata
Title
CCK1 receptor is involved in the regulation of protein lysine acetylation in GBC-SD cells and gallbladder carcinoma
Authors
W. Wu
B. Ouyang
Z. Lu
H. Liu
Y. Tan
P. Cui
Publication date
01-11-2017
Publisher
Springer London
Published in
Irish Journal of Medical Science (1971 -) / Issue 4/2017
Print ISSN: 0021-1265
Electronic ISSN: 1863-4362
DOI
https://doi.org/10.1007/s11845-017-1603-2

Other articles of this Issue 4/2017

Irish Journal of Medical Science (1971 -) 4/2017 Go to the issue

Original Article

Five year trends in maternal smoking behaviour reported at the first prenatal appointment

Editorial

In recognition of Ireland’s contemporary medical scientists—Ted Dinan, Timothy O’Brien, Michael Hutchinson, Desmond FitzGerald, Michael Gibney, Mary Cannon and Fergal O’Gara

Original Article

Dynamic sentinel node biopsy for penile cancer: an initial experience in an Irish Hospital

Original Article

Circulating rotavirus genotypes in the Irish paediatric population prior to the introduction of the vaccination programme

Original Article

The analysis of lncRNA HOTAIR rs12826786 C>T polymorphism and gastric cancer susceptibility in a Turkish population: lack of any association in a hospital-based case–control study

Original Article

Effects of remote ischemic preconditioning on acute myocardial injury in patients undergoing valve replacement
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits