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Irish Journal of Medical Science (1971 -)

Published in:

01-08-2017 | Original Article

Obesity is not a descriptive factor for oxidative stress and viscosity in follicular fluid of in vitro fertilization patients

Authors: B. Yuksel, S. Kilic, N. Yilmaz, T. Goktas, U. Keskin, A. Seven, M. Ulubay, S. Batioglu

Published in: Irish Journal of Medical Science (1971 -) | Issue 3/2017

Abstract

Background

Obesity’s impact on micro-environmental oxidative stress and follicular fluid (FF) viscosity and whether or not it has any effect on in vitro fertilization (IVF) success is a matter of debate.

Aims

In this study, our aim was to evaluate the levels of oxidative stress markers and the FF viscosity in obese and non-obese patients.

Methods

Eighty norm-responder patients undergoing IVF were prospectively grouped according to their body mass indexes (BMI). Group 1 (n = 40) and group 2 (n = 40) had BMI values of ≤24.9 and ≥25.0, respectively. Total sulfhydryl (RSH) levels (nmol/m) and the formation of thiobarbituric acid-reactive substances (malondialdehyde, or MDA) (µmol/ml) in FFs were quantified. For the first time in our study, FF viscosity with changing BMI values was also determined.

Results

The mean levels of MDA (µmol/ml) and RSH (nmol/ml) were not significantly different between groups (1.37 ± 0.51; 1.51 ± 0.51; p > 0.05 for MDA and 0.42 ± 0.30; 0.41 ± 0.20; p > 0.05 for RSH, respectively). Similarly, the FF viscosity (centipoise) was not different between groups (1.28 ± 0.28; 1.30 ± 0.19; p < 0.05, respectively). Independent of BMI, no correlation was found between FF levels of oxidative markers and the number of oocytes retrieved or the fertilization rates.

Conclusions

In our study, we found no difference in the levels of follicular oxidative and anti-oxidative markers or the follicular fluid viscosity with changing BMI values. We also demonstrated that the levels of oxidative stress markers and the viscosity of follicular fluid did not affect clinical outcomes.

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Title: Obesity is not a descriptive factor for oxidative stress and viscosity in follicular fluid of in vitro fertilization patients
Authors: B. Yuksel
S. Kilic
N. Yilmaz
T. Goktas
U. Keskin
A. Seven
M. Ulubay
S. Batioglu
Publication date: 01-08-2017
Publisher: Springer London
Published in: Irish Journal of Medical Science (1971 -) / Issue 3/2017
Print ISSN: 0021-1265
Electronic ISSN: 1863-4362
DOI: https://doi.org/10.1007/s11845-016-1523-6

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Conclusions

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