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Obesity Surgery
Published in: Obesity Surgery 11/2018

01-11-2018 | Original Contributions

No Difference in Ghrelin-Producing Cell Expression in Obese Versus Non-obese Stomach: a Prospective Histopathological Case-Control Study

Authors: Mario Musella, Francesco Di Capua, Maria D’Armiento, Nunzio Velotti, Alessio Bocchetti, Katia Di Lauro, Giuseppe Galloro, Severo Campione, Giuseppe Petrella, Francesco Paolo D’Armiento

Published in: Obesity Surgery | Issue 11/2018

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Abstract

Background

To understand the role of ghrelin in the mechanism of action of laparoscopic sleeve gastrectomy (LSG), a prospective cohort case-control study to assess the expression of ghrelin-producing cells (GPC) in two groups of patients was designed.

Methods

Specimens of resected stomach from 26 obese patients who underwent LSG (group A), were compared by immunohistochemistry to control stomach samples from 26 non-obese patients (group B) resected for other pathologies or during autopsy; (GIST: 6 cases, inflammatory diseases: 4 cases, post-mortem autopsy cases with stomachs from healthy persons victims of traumatic accidents: 16 cases). Immunohistochemistry investigation was performed with the use of Ventana Benchmark ultra, anti-ghrelin antibody NOVUS, mouse monoclonal 2F4, diluted at 1:100.

Results

No significant difference in the expression of GPC number between group A and B was found (p = 0.87). No significant correlation between patients presenting a GPC number above (subgroup 1) or below (subgroup 2) the average, and EWL% changes, both at 1 and 6 years of follow-up, was recorded.

Conclusions

Our study has shown that the expression of GPC is similar in the stomach of obese and non-obese controls, being mostly influenced by the inflammatory status of the gastric mucosa. A variation in the preoperative number of GPC has not influenced the weight loss in patients who underwent LSG.
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Metadata
Title
No Difference in Ghrelin-Producing Cell Expression in Obese Versus Non-obese Stomach: a Prospective Histopathological Case-Control Study
Authors
Mario Musella
Francesco Di Capua
Maria D’Armiento
Nunzio Velotti
Alessio Bocchetti
Katia Di Lauro
Giuseppe Galloro
Severo Campione
Giuseppe Petrella
Francesco Paolo D’Armiento
Publication date
01-11-2018
Publisher
Springer US
Published in
Obesity Surgery / Issue 11/2018
Print ISSN: 0960-8923
Electronic ISSN: 1708-0428
DOI
https://doi.org/10.1007/s11695-018-3401-y

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