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Obesity Surgery
Published in: Obesity Surgery 10/2018

Open Access 01-10-2018 | Original Contributions

Roux-en-Y Gastric Bypass Surgery-Induced Weight Loss and Metabolic Improvements Are Similar in TGR5-Deficient and Wildtype Mice

Authors: Zheng Hao, R. Leigh Townsend, Michael B. Mumphrey, Thomas W. Gettys, Sangho Yu, Heike Münzberg, Christopher D. Morrison, Hans-Rudolf Berthoud

Published in: Obesity Surgery | Issue 10/2018

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Abstract

Background and Purpose

Roux-en-Y gastric bypass surgery (RYGB) remains one of the most effective treatments for obesity and type 2 diabetes. Despite this, the mechanisms through which it acts are still not well understood. Bile acid signaling through the transmembrane G-protein-coupled receptor TGR5 has been shown to have significant effects on metabolism and has recently been reported to be necessary for the full effects of vertical sleeve gastrectomy (VSG), a bariatric surgery with similar effects to RYGB. The goal of the current study is therefore to investigate the role of bile acid signaling through TGR5 to see if it is necessary to obtain the full effects of RYGB.

Methods

High-fat diet-induced obese TGR5−/− and wildtype mice (WT) were subjected to RYGB, sham surgery, or weight matching (WM) to RYGB mice via caloric restriction. Body weight, body composition, food intake, energy expenditure, glucose tolerance, insulin sensitivity, and liver weight were measured.

Results

Although the difference in fat mass 20 weeks after surgery between RYGB and sham-operated mice was slightly reduced in TGR5−/− mice when compared to wildtype mice, loss of body weight and fat mass from preoperative levels, reduction of food intake, increase of energy expenditure, and improvement in glycemic control were similar in the two genotypes. Furthermore, improvements in glycemic control were similar in non-surgical mice weight-matched to RYGB.

Conclusions

We conclude that bile acid signaling through TGR5 is not required for the beneficial effects of RYGB in the mouse and that RYGB and VSG may achieve their similar beneficial effects through different mechanisms.
Appendix
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Metadata
Title
Roux-en-Y Gastric Bypass Surgery-Induced Weight Loss and Metabolic Improvements Are Similar in TGR5-Deficient and Wildtype Mice
Authors
Zheng Hao
R. Leigh Townsend
Michael B. Mumphrey
Thomas W. Gettys
Sangho Yu
Heike Münzberg
Christopher D. Morrison
Hans-Rudolf Berthoud
Publication date
01-10-2018
Publisher
Springer US
Published in
Obesity Surgery / Issue 10/2018
Print ISSN: 0960-8923
Electronic ISSN: 1708-0428
DOI
https://doi.org/10.1007/s11695-018-3297-6

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