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Journal of Gastrointestinal Surgery

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01-01-2020 | Ileus | 2019 SSAT Plenary Presentation

Burn-Induced Impairment of Ileal Muscle Contractility Is Associated with Increased Extracellular Matrix Components

Authors: Claire B. Cummins, Yanping Gu, Xiaofu Wang, You-Min Lin, Xuan-Zheng Shi, Ravi S. Radhakrishnan

Published in: Journal of Gastrointestinal Surgery | Issue 1/2020

Abstract

Introduction

Severe burns lead to marked impairment of gastrointestinal motility, such as delayed gastric emptying and small and large intestinal ileus. However, the cellular mechanism of these pathologic changes remains largely unknown.

Methods

Male Sprague Dawley rats approximately 3 months old and weighing 300–350 g were randomized to either a 60% total body surface area full-thickness scald burn or sham procedure and were sacrificed 24 h after the procedure. Gastric emptying, gastric antrum contractility ileal smooth muscle contractility, and colonic contractility were measured. Muscularis externa was isolated from the ileal segment to prepare smooth muscle protein extracts for Western blot analysis.

Results

Compared with sham controls, the baseline rhythmic contractile activities of the antral, ileal, and colonic smooth muscle strips were impaired in the burned rats. Simultaneously, our data showed that ileal muscularis ECM proteins fibronectin and laminin were significantly up-regulated in burned rats compared with sham rats. TGF-β signaling is an important stimulating factor for ECM protein expression. Our results revealed that TGF-β signaling was activated in the ileal muscle of burned rats evidenced by the activation of Smad2/3 expression and phosphorylation. In addition, the total and phosphorylated AKT, which is an important downstream factor of ECM signaling in smooth muscle cells, was also up-regulated in burned rats’ ileal muscle. Notably, these changes were not seen in the colonic or gastric tissues.

Conclusion

Deposition of fibrosis-related proteins after severe burn is contributors to decreased small intestinal motility.

Alican I, Coskun T, Yegen C, Aktan AO, Yalin R, Yegen BC. The effect of thermal injury on gastric emptying in rats. Burns 1995;21(3):171–4.CrossRef

Czaja AJ, McAlhany JC, Pruitt BA, Jr. Acute gastroduodenal disease after thermal injury. An endoscopic evaluation of incidence and natural history. N Engl J Med 1974;291(18):925–9. doi:https://doi.org/10.1056/NEJM197410312911801.CrossRefPubMed

Chen CF, Chapman BJ, Munday KA, Fang HS. The effects of thermal injury on gastrointestinal motor activity in the rat. Burns Incl Therm Inj 1982;9(2):142–6.CrossRef

Unluer EE, Alican I, Yegen C, Yegen BC. The delays in intestinal motility and neutrophil infiltration following burn injury in rats involve endogenous endothelins. Burns 2000;26(4):335–40.CrossRef

Wolf SE, Ikeda H, Matin S, Debroy MA, Rajaraman S, Herndon DN et al. Cutaneous burn increases apoptosis in the gut epithelium of mice. J Am Coll Surg 1999;188(1):10–6.CrossRef

Huang HH, Lee YC, Chen CY. Effects of burns on gut motor and mucosa functions. Neuropeptides 2018;72:47–57. doi:https://doi.org/10.1016/j.npep.2018.09.004.CrossRefPubMed

Oliveira HM, Sallam HS, Espana-Tenorio J, Chinkes D, Chung DH, Chen JD et al. Gastric and small bowel ileus after severe burn in rats: the effect of cyclooxygenase-2 inhibitors. Burns 2009;35(8):1180–4. doi:https://doi.org/10.1016/j.burns.2009.02.022.CrossRefPubMed

Sallam HS, Oliveira HM, Liu S, Chen JD. Mechanisms of burn-induced impairment in gastric slow waves and emptying in rats. Am J Physiol Regul Integr Comp Physiol 2010;299(1):R298–305. doi:https://doi.org/10.1152/ajpregu.00135.2010.CrossRefPubMed

Sahu K, Kaurav M, Pandey RS. Protease loaded permeation enhancer liposomes for treatment of skin fibrosis arisen from second degree burn. Biomed Pharmacother 2017;94:747–57. doi:https://doi.org/10.1016/j.biopha.2017.07.141.CrossRefPubMed

10.

Sokhn S, Nasseh I. Dermal fibrosis and calcification secondary to burn injury. Quintessence Int 2009;40(6):503–6.PubMed

11.

Gabriel VA. Transforming growth factor-beta and angiotensin in fibrosis and burn injuries. J Burn Care Res 2009;30(3):471–81. doi:https://doi.org/10.1097/BCR.0b013e3181a28ddb.CrossRefPubMed

12.

Ulrich D, Noah EM, von Heimburg D, Pallua N. TIMP-1, MMP-2, MMP-9, and PIIINP as serum markers for skin fibrosis in patients following severe burn trauma. Plast Reconstr Surg 2003;111(4):1423–31. doi:https://doi.org/10.1097/01.PRS.0000049450.95669.07.CrossRefPubMed

13.

Duke JM, Randall SM, Fear MW, Boyd JH, Rea S, Wood FM. Long-term Effects of Pediatric Burns on the Circulatory System. Pediatrics 2015;136(5):e1323–30. doi:https://doi.org/10.1542/peds.2015-1945.CrossRefPubMed

14.

Duke JM, Randall SM, Fear MW, Boyd JH, Rea S, Wood FM. Understanding the long-term impacts of burn on the cardiovascular system. Burns 2016;42(2):366–74. doi:https://doi.org/10.1016/j.burns.2015.08.020.CrossRefPubMed

15.

Hundeshagen G, Herndon DN, Clayton RP, Wurzer P, McQuitty A, Jennings K et al. Long-term effect of critical illness after severe paediatric burn injury on cardiac function in adolescent survivors: an observational study. Lancet Child Adolesc Health 2017;1(4):293–301. doi:https://doi.org/10.1016/S2352-4642(17)30122-0.CrossRefPubMedPubMedCentral

16.

Jeschke MG, Micak RP, Finnerty CC, Herndon DN. Changes in liver function and size after a severe thermal injury. Shock 2007;28(2):172–7. doi:https://doi.org/10.1097/shk.0b013e318047b9e2.CrossRefPubMed

17.

Price LA, Thombs B, Chen CL, Milner SM. Liver disease in burn injury: evidence from a national sample of 31,338 adult patients. J Burns Wounds 2007;7:e1.PubMedPubMedCentral

18.

Peyton SR, Kim PD, Ghajar CM, Seliktar D, Putnam AJ. The effects of matrix stiffness and RhoA on the phenotypic plasticity of smooth muscle cells in a 3-D biosynthetic hydrogel system. Biomaterials 2008;29(17):2597–607. doi:https://doi.org/10.1016/j.biomaterials.2008.02.005.CrossRefPubMedPubMedCentral

19.

Peyton SR, Putnam AJ. Extracellular matrix rigidity governs smooth muscle cell motility in a biphasic fashion. J Cell Physiol 2005;204(1):198–209. doi:https://doi.org/10.1002/jcp.20274.CrossRefPubMed

20.

Herrick WG, Rattan S, Nguyen TV, Grunwald MS, Barney CW, Crosby AJ et al. Smooth Muscle Stiffness Sensitivity is Driven by Soluble and Insoluble ECM Chemistry. Cell Mol Bioeng 2015;8(3):333–48. doi:https://doi.org/10.1007/s12195-015-0397-4.CrossRefPubMedPubMedCentral

21.

Gaudet C, Marganski WA, Kim S, Brown CT, Gunderia V, Dembo M et al. Influence of type I collagen surface density on fibroblast spreading, motility, and contractility. Biophys J 2003;85(5):3329–35. doi:https://doi.org/10.1016/S0006-3495(03)74752-3.CrossRefPubMedPubMedCentral

22.

Scheibe K, Kersten C, Schmied A, Vieth M, Primbs T, Carle B et al. Inhibiting Interleukin 36 Receptor Signaling Reduces Fibrosis in Mice With Chronic Intestinal Inflammation. Gastroenterology 2019;156(4):1082–97 e11. doi:https://doi.org/10.1053/j.gastro.2018.11.029.CrossRefPubMed

23.

Rieder F, Fiocchi C, Rogler G. Mechanisms, Management, and Treatment of Fibrosis in Patients With Inflammatory Bowel Diseases. Gastroenterology 2017;152(2):340–50 e6. doi:https://doi.org/10.1053/j.gastro.2016.09.047.CrossRefPubMed

24.

Mascarenhas DD, Elayadi A, Singh BK, Prasai A, Hegde SD, Herndon DN et al. Nephrilin peptide modulates a neuroimmune stress response in rodent models of burn trauma and sepsis. Int J Burns Trauma 2013;3(4):190–200.PubMedPubMedCentral

25.

Bohanon FJ, Nunez Lopez O, Herndon DN, Wang X, Bhattarai N, Ayadi AE et al. Burn Trauma Acutely Increases the Respiratory Capacity and Function of Liver Mitochondria. Shock 2018;49(4):466–73. doi:https://doi.org/10.1097/SHK.0000000000000935.CrossRefPubMedPubMedCentral

26.

Shi XZ, Lin YM, Powell DW, Sarna SK. Pathophysiology of motility dysfunction in bowel obstruction: role of stretch-induced COX-2. Am J Physiol Gastrointest Liver Physiol 2011;300(1):G99-G108. doi:https://doi.org/10.1152/ajpgi.00379.2010.CrossRefPubMed

27.

Lin YM, Fu Y, Winston J, Radhakrishnan R, Sarna SK, Huang LM et al. Pathogenesis of abdominal pain in bowel obstruction: role of mechanical stress-induced upregulation of nerve growth factor in gut smooth muscle cells. Pain 2017;158(4):583–92. doi:https://doi.org/10.1097/j.pain.0000000000000797.CrossRefPubMedPubMedCentral

28.

Hegde S, Lin YM, Golovko G, Khanipov K, Cong Y, Savidge T et al. Microbiota dysbiosis and its pathophysiological significance in bowel obstruction. Sci Rep 2018;8(1):13044. doi:https://doi.org/10.1038/s41598-018-31033-0.CrossRefPubMedPubMedCentral

29.

Li H, Yin J, Zhang Z, Winston JH, Shi XZ, Chen JD. Auricular vagal nerve stimulation ameliorates burn-induced gastric dysmotility via sympathetic-COX-2 pathways in rats. Neurogastroenterol Motil 2016;28(1):36–42. doi:https://doi.org/10.1111/nmo.12693.CrossRefPubMed

30.

Cummins CB, Wang X, Nunez Lopez O, Graham G, Tie HY, Zhou J et al. Luteolin-Mediated Inhibition of Hepatic Stellate Cell Activation via Suppression of the STAT3 Pathway. Int J Mol Sci 2018;19(6). doi:https://doi.org/10.3390/ijms19061567.CrossRef

31.

Cummins CB, Wang X, Sommerhalder C, Bohanon FJ, Nunez Lopez O, Tie HY et al. Natural Compound Oridonin Inhibits Endotoxin-Induced Inflammatory Response of Activated Hepatic Stellate Cells. Biomed Res Int 2018;2018:6137420. doi:https://doi.org/10.1155/2018/6137420.CrossRefPubMedPubMedCentral

32.

Cummins CB, Wang X, Xu J, Hughes BD, Ding Y, Chen H et al. Antifibrosis Effect of Novel Oridonin Analog CYD0618 Via Suppression of the NF-kappaB Pathway. J Surg Res 2018;232:283–92. doi:https://doi.org/10.1016/j.jss.2018.06.040.CrossRefPubMedPubMedCentral

33.

Sallam HS, Kramer GC, Chen JD. Gastric emptying and intestinal transit of various enteral feedings following severe burn injury. Dig Dis Sci 2011;56(11):3172–8. doi:https://doi.org/10.1007/s10620-011-1755-2.CrossRefPubMed

34.

Sallam HS, Oliveira HM, Gan HT, Herndon DN, Chen JD. Ghrelin improves burn-induced delayed gastrointestinal transit in rats. Am J Physiol Regul Integr Comp Physiol 2007;292(1):R253–7. doi:https://doi.org/10.1152/ajpregu.00100.2006.CrossRefPubMed

35.

Gan HT, Chen JD. Roles of nitric oxide and prostaglandins in pathogenesis of delayed colonic transit after burn injury in rats. Am J Physiol Regul Integr Comp Physiol 2005;288(5):R1316–24. doi:https://doi.org/10.1152/ajpregu.00733.2004.CrossRefPubMed

36.

Oktar BK, Cakir B, Mutlu N, Celikel C, Alican I. Protective role of cyclooxygenase (COX) inhibitors in burn-induced intestinal and liver damage. Burns 2002;28(3):209–14.CrossRef

37.

Duband JL, Gimona M, Scatena M, Sartore S, Small JV. Calponin and SM 22 as differentiation markers of smooth muscle: spatiotemporal distribution during avian embryonic development. Differentiation 1993;55(1):1–11.CrossRef

38.

Rokolya A, Ahn HY, Moreland S, van Breemen C, Moreland RS. A hypothesis for the mechanism of receptor and G-protein-dependent enhancement of vascular smooth muscle myofilament Ca2+ sensitivity. Can J Physiol Pharmacol 1994;72(11):1420–6.CrossRef

39.

Shimomura E, Shiraishi M, Iwanaga T, Seto M, Sasaki Y, Ikeda M et al. Inhibition of protein kinase C-mediated contraction by Rho kinase inhibitor fasudil in rabbit aorta. Naunyn Schmiedebergs Arch Pharmacol 2004;370(5):414–22. doi:https://doi.org/10.1007/s00210-004-0975-9.CrossRefPubMed

40.

Sohn UD, Cao W, Tang DC, Stull JT, Haeberle JR, Wang CL et al. Myosin light chain kinase- and PKC-dependent contraction of LES and esophageal smooth muscle. Am J Physiol Gastrointest Liver Physiol 2001;281(2):G467–78. doi:https://doi.org/10.1152/ajpgi.2001.281.2.G467.CrossRefPubMed

41.

Ringvold HC, Khalil RA. Protein Kinase C as Regulator of Vascular Smooth Muscle Function and Potential Target in Vascular Disorders. Adv Pharmacol 2017;78:203–301. doi:https://doi.org/10.1016/bs.apha.2016.06.002.CrossRefPubMed

42.

Uray KS, Laine GA, Xue H, Allen SJ, Cox CS, Jr. Intestinal edema decreases intestinal contractile activity via decreased myosin light chain phosphorylation. Crit Care Med 2006;34(10):2630–7. doi:https://doi.org/10.1097/01.CCM.0000239195.06781.8C.CrossRefPubMed

43.

Touyz RM, Alves-Lopes R, Rios FJ, Camargo LL, Anagnostopoulou A, Arner A et al. Vascular smooth muscle contraction in hypertension. Cardiovasc Res 2018;114(4):529–39. doi:https://doi.org/10.1093/cvr/cvy023.CrossRefPubMedPubMedCentral

44.

Hruz P, Dann SM, Eckmann L. STAT3 and its activators in intestinal defense and mucosal homeostasis. Curr Opin Gastroenterol 2010;26(2):109–15. doi:https://doi.org/10.1097/MOG.0b013e3283365279.CrossRefPubMed

45.

Xiao K, Song ZH, Jiao LF, Ke YL, Hu CH. Developmental changes of TGF-beta1 and Smads signaling pathway in intestinal adaption of weaned pigs. PLoS One 2014;9(8):e104589. doi:https://doi.org/10.1371/journal.pone.0104589.CrossRefPubMedPubMedCentral

46.

Tinoco-Veras CM, Santos A, Stipursky J, Meloni M, Araujo APB, Foschetti DA et al. Transforming Growth Factor beta1/SMAD Signaling Pathway Activation Protects the Intestinal Epithelium from Clostridium difficile Toxin A-Induced Damage. Infect Immun 2017;85(10). doi:https://doi.org/10.1128/IAI.00430-17.

47.

Mann ER, Bernardo D, English NR, Landy J, Al-Hassi HO, Peake ST et al. Compartment-specific immunity in the human gut: properties and functions of dendritic cells in the colon versus the ileum. Gut 2016;65(2):256–70. doi:https://doi.org/10.1136/gutjnl-2014-307916.CrossRefPubMed

48.

Santaolalla R, Fukata M, Abreu MT. Innate immunity in the small intestine. Curr Opin Gastroenterol 2011;27(2):125–31. doi:https://doi.org/10.1097/MOG.0b013e3283438dea.CrossRefPubMedPubMedCentral

49.

Farina JA, Jr., Rosique MJ, Rosique RG. Curbing inflammation in burn patients. Int J Inflam 2013;2013:715645. doi:https://doi.org/10.1155/2013/715645.CrossRefPubMedPubMedCentral

50.

Latella G, Rieder F. Intestinal fibrosis: ready to be reversed. Curr Opin Gastroenterol 2017;33(4):239–45. doi:https://doi.org/10.1097/MOG.0000000000000363.CrossRefPubMedPubMedCentral

51.

Wang Z, Li R, Zhong R. Extracellular matrix promotes proliferation, migration and adhesion of airway smooth muscle cells in a rat model of chronic obstructive pulmonary disease via upregulation of the PI3K/AKT signaling pathway. Mol Med Rep 2018;18(3):3143–52. doi:https://doi.org/10.3892/mmr.2018.9320.CrossRefPubMedPubMedCentral

52.

Wen JJ, Radhakrishnan GL, Cummins CB, Radhakrishnan RS. Sildenafil Prevents Adverse Cardiac Remodeling and LV Dysfunction in an In Vivo Model of Burn Injury. J Burn Care Res 2019;40(S1):S27.CrossRef

Title: Burn-Induced Impairment of Ileal Muscle Contractility Is Associated with Increased Extracellular Matrix Components
Authors: Claire B. Cummins
Yanping Gu
Xiaofu Wang
You-Min Lin
Xuan-Zheng Shi
Ravi S. Radhakrishnan
Publication date: 01-01-2020
Publisher: Springer US
Keyword: Ileus
Published in: Journal of Gastrointestinal Surgery / Issue 1/2020
Print ISSN: 1091-255X
Electronic ISSN: 1873-4626
DOI: https://doi.org/10.1007/s11605-019-04400-z

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Burn-Induced Impairment of Ileal Muscle Contractility Is Associated with Increased Extracellular Matrix Components

Abstract

Introduction

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Introduction

Methods

Results

Conclusion

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