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Journal of Gastrointestinal Surgery

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01-06-2009 | Original Article

Esophagus Tissue Engineering: Hybrid Approach with Esophageal Epithelium and Unidirectional Smooth Muscle Tissue Component Generation In Vitro

Authors: Amulya K. Saxena, Kristina Kofler, Herwig Ainödhofer, Micheal E. Höllwarth

Published in: Journal of Gastrointestinal Surgery | Issue 6/2009

Abstract

Purpose

The aim of this study was to engineer the two main components of the esophagus in vitro: (a) esophageal epithelium and (b) smooth muscle tissue. Furthermore, (a) survivability of esophageal epithelial cells (EEC) on basement membrane matrix (BMM)-coated scaffolds and (b) oriented smooth muscle tissue formation on unidirectional BMM-coated collagen scaffolds was investigated.

Methods

Both EEC and smooth muscle cells (SMC) were sourced from Sprague–Dawley rats. The EEC were maintained in vitro and seeded onto BMM-coated 2-D collagen scaffolds. Similarly, smooth muscle cells were obtained using an explants technique and seeded on unidirectional 3-D BMM-coated collagen scaffolds. Cell–polymer constructs for EEC and SMC were maintained in vitro for 8 weeks.

Results

Protocols to obtain higher yield of EEC were established. EEC formed a layer of differentiated epithelium after 14 days. EEC survivability on polymers was observed up to 8 weeks. Unidirectional smooth muscle tissue strands were successfully engineered.

Conclusion

Esophageal epithelium generation, survivability of EEC on BMM-coated scaffolds, and engineering of unidirectional smooth muscle strands were successful in vitro. The hybrid approach of assembling individual tissue components in vitro using BMM-coated scaffolds and later amalgamating them to form composite tissue holds promises in the tissue engineering of complex organ systems.

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Title: Esophagus Tissue Engineering: Hybrid Approach with Esophageal Epithelium and Unidirectional Smooth Muscle Tissue Component Generation In Vitro
Authors: Amulya K. Saxena
Kristina Kofler
Herwig Ainödhofer
Micheal E. Höllwarth
Publication date: 01-06-2009
Publisher: Springer-Verlag
Published in: Journal of Gastrointestinal Surgery / Issue 6/2009
Print ISSN: 1091-255X
Electronic ISSN: 1873-4626
DOI: https://doi.org/10.1007/s11605-009-0836-4

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Esophagus Tissue Engineering: Hybrid Approach with Esophageal Epithelium and Unidirectional Smooth Muscle Tissue Component Generation In Vitro

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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