Top

Published in:

01-07-2012 | Original Article

Hepatocyte transporter expression in FNH and FNH-like nodule: correlation with signal intensity on gadoxetic acid enhanced magnetic resonance images

Authors: Norihide Yoneda, Osamu Matsui, Azusa Kitao, Ryuuichi Kita, Kazuto Kozaka, Wataru Koda, Satoshi Kobayashi, Toshifumi Gabata, Hiroko Ikeda, Yasunori Sato, Yasuni Nakanuma

Published in: Japanese Journal of Radiology | Issue 6/2012

Abstract

Purpose

Our aim was to evaluate the hepatocyte transporters in focal nodular hyperplasia (FNH) and FNH-like lesions and to correlate the grade of its expression with signal intensity on the hepatobiliary phase (HB phase) of gadoxetic-acid-enhanced magnetic resonance imaging (EOB-MRI).

Materials and methods

Thirteen histopathological confirmed cases including eight with EOB-MRI were studied. Immunohistochemical staining for transporter was performed and its grade semiquantitatively analyzed.

Results

Histopathologically, ten cases showed almost equal organic anion transporter polypeptide (OATP) 8 expression relative to the surrounding liver; the remaining three showed stronger OATP8 expression. In eight cases with EOB-MRI, two demonstrated more hyperintensity on the HB phase, and their OATP8 expression was stronger compared with the surrounding liver. The remaining six cases showed isointensity on the HB phase and revealed almost equal OATP8 expression. The expression of export transporter multi-drug-resistant proteins (MRP) 1 and 2 were almost equal relative to the surrounding liver in most cases (11/12, 92 %; 11/12, 92 %, respectively), whereas MRP3 focally overexpressed in 75 % (9/12) of cases.

Conclusion

FNH and FNH-like nodules revealed equal or stronger OATP8 expression than background liver. OATP8 expression showed significant correlation with signal intensity on the HB phase.

Vogl T, Kümmel S, Hammerstingl R, Schellenbeck M, Schumacher G, Balzer T, et al. Liver tumors: comparison of MR imaging with Gd-EOB-DTPA and Gd-DTPA. Radiology. 1996;200(1):59–67.PubMed

Bluemke D, Sahani D, Amendola M, Balzer T, Breuer J, Brown JJ, et al. Efficacy and safety of MR imaging with liver-specific contrast agent: U.S. multicenter phase III study. Radiology. 2005;237(1):89–98.PubMedCrossRef

Reimer P, Schneider G, Schima W. Hepatobiliary contrast agents for contrast-enhanced MRI of the liver: properties, clinical development and applications. Eur Radiol. 2004;14(4):559–78.PubMedCrossRef

Schuhmann-Giampieri G, Schmitt-Willich H, Press W, Negishi C, Weinmann H, Speck U. Preclinical evaluation of Gd-EOB-DTPA as a contrast agent in MR imaging of the hepatobiliary system. Radiology. 1992;183(1):59–64.PubMed

Huppertz A, Haraida S, Kraus A, Zech CJ, Scheidler J, Breuer J, et al. Enhancement of focal liver lesions at gadoxetic acid-enhanced MR imaging: correlation with histopathologic findings and spiral CT—initial observations. Radiology. 2005;234(2):468–78.PubMedCrossRef

Hamm B, Staks T, Mühler A, Bollow M, Taupitz M, Frenzel T, et al. Phase I clinical evaluation of Gd-EOB-DTPA as a hepatobiliary MR contrast agent: safety, pharmacokinetics, and MR imaging. Radiology. 1995;195(3):785–92.PubMed

Reimer P, Rummeny E, Shamsi K, Balzer T, Daldrup HE, Tombach B, et al. Phase II clinical evaluation of Gd-EOB-DTPA: dose, safety aspects, and pulse sequence. Radiology. 1996;199(1):177–83.PubMed

Zech C, Grazioli L, Breuer J, Reiser M, Schoenberg S. Diagnostic performance and description of morphological features of focal nodular hyperplasia in Gd-EOB-DTPA-enhanced liver magnetic resonance imaging: results of a multicenter trial. Invest Radiol. 2008;43(7):504–11.PubMedCrossRef

Vander Borght S, Libbrecht L, Blokzijl H, Faber KN, Moshage H, Aerts R, et al. Diagnostic and pathogenetic implications of the expression of hepatic transporters in focal lesions occurring in normal liver. J Pathol. 2005;207(4):471–82.PubMedCrossRef

10.

Nakanuma Y. Non-neoplastic nodular lesions in the liver. Pathol Int. 1995;45(10):703–14.PubMedCrossRef

11.

Terminology of Nodular Hepatocellular Lesions. International working party. Hepatology. 1995;22(3):983–93.

12.

Bioulac-Sage P, Balabaud C, Bedossa P, Scoazec JY, Chiche L, Dhillon AP, et al. Pathological diagnosis of liver cell adenoma and focal nodular hyperplasia: Bordeaux update. J Hepatol. 2007;46(3):521–7.PubMedCrossRef

13.

Sugihara S, Nakashima O, Kiyomatsu K, Ijiri M, Edamitsu O, Kojiro M. A case of liver cirrhosis with a hyperplastic nodular lesion. Acta Pathol Jpn. 1990;40(9):699–703.PubMed

14.

Terada T, Kitani S, Ueda K, Nakanuma Y, Kitagawa K, Masuda S. Adenomatous hyperplasia of the liver resembling focal nodular hyperplasia in patients with chronic liver disease. Virchows Arch A Pathol Anat Histopathol. 1993;422(3):247–52.PubMedCrossRef

15.

Quaglia A, Tibballs J, Grasso A, Prasad N, Nozza P, Davies SE, et al. Focal nodular hyperplasia-like areas in cirrhosis. Histopathology. 2003;42(1):14–21.PubMedCrossRef

16.

An H, Illei P, Diflo T, John D, Morgan G, Teperman L, et al. Scirrhous changes in dysplastic nodules do not indicate high-grade status. J Gastroenterol Hepatol. 2003;18(6):660–5.PubMedCrossRef

17.

Nakashima O, Kurogi M, Yamaguchi R, Miyaaki H, Fujimoto M, Yano H, et al. Unique hypervascular nodules in alcoholic liver cirrhosis: identical to focal nodular hyperplasia-like nodules? J Hepatol. 2004;41(6):992–8.PubMedCrossRef

18.

Wanless I, Mawdsley C, Adams R. On the pathogenesis of focal nodular hyperplasia of the liver. Hepatology. 1985;5(6):1194–200.PubMedCrossRef

19.

Bioulac-Sage P, Balabaud C, Wanless I. Diagnosis of focal nodular hyperplasia: not so easy. Am J Surg Pathol. 2001;25(10):1322–5.PubMedCrossRef

20.

Welch T, Sheedy PF 2nd, Johnson C, Stephens DH, Charboneau JW, Brown ML, et al. Focal nodular hyperplasia and hepatic adenoma: comparison of angiography, CT, US, and scintigraphy. Radiology. 1985;156(3):593–5.PubMed

21.

Carlson S, Johnson C, Bender C, Welch T. CT of focal nodular hyperplasia of the liver. AJR Am J Roentgenol. 2000;174(3):705–12.PubMed

22.

Brancatelli G, Federle M, Grazioli L, Blachar A, Peterson M, Thaete L. Focal nodular hyperplasia: CT findings with emphasis on multiphasic helical CT in 78 patients. Radiology. 2001;219(1):61–8.PubMed

23.

Miyayama S, Matsui O, Ueda K, Kifune K, Yamashiro M, Yamamoto T, et al. Hemodynamics of small hepatic focal nodular hyperplasia: evaluation with single-level dynamic CT during hepatic arteriography. AJR Am J Roentgenol. 2000;174(6):1567–9.PubMed

24.

Giovanoli O, Heim M, Terracciano L, Bongartz G, Ledermann HP. MRI of hepatic adenomatosis: initial observations with gadoxetic acid contrast agent in three patients. AJR Am J Roentgenol. 2008;190(5):W290–3.PubMedCrossRef

25.

Kitao A, Zen Y, Matsui O, Gabata T, Kobayashi S, Koda W, et al. Hepatocellular carcinoma: signal intensity at gadoxetic acid-enhanced MR imaging—correlation with molecular transporters and histopathologic features. Radiology. 2010;256(3):817–26.PubMedCrossRef

26.

Leonhardt M, Keiser M, Oswald S, Kühn J, Jia J, Grube M, et al. Hepatic uptake of the magnetic resonance imaging contrast agent Gd-EOB-DTPA: role of human organic anion transporters. Drug Metab Dispos. 2010;38(7):1024–8.PubMedCrossRef

27.

Narita M, Hatano E, Arizono S, Miyagawa-Hayashino A, Isoda H, Kitamura K, et al. Expression of OATP1B3 determines uptake of Gd-EOB-DTPA in hepatocellular carcinoma. J Gastroenterol. 2009;44(7):793–8.PubMedCrossRef

28.

Saito K, Kotake F, Ito N, Ozuki T, Mikami R, Abe K, et al. Gd-EOB-DTPA enhanced MRI for hepatocellular carcinoma: quantitative evaluation of tumor enhancement in hepatobiliary phase. Magn Reson Med Sci. 2005;4(1):1–9.PubMedCrossRef

Title: Hepatocyte transporter expression in FNH and FNH-like nodule: correlation with signal intensity on gadoxetic acid enhanced magnetic resonance images
Authors: Norihide Yoneda
Osamu Matsui
Azusa Kitao
Ryuuichi Kita
Kazuto Kozaka
Wataru Koda
Satoshi Kobayashi
Toshifumi Gabata
Hiroko Ikeda
Yasunori Sato
Yasuni Nakanuma
Publication date: 01-07-2012
Publisher: Springer Japan
Published in: Japanese Journal of Radiology / Issue 6/2012
Print ISSN: 1867-1071
Electronic ISSN: 1867-108X
DOI: https://doi.org/10.1007/s11604-012-0085-4

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Hepatocyte transporter expression in FNH and FNH-like nodule: correlation with signal intensity on gadoxetic acid enhanced magnetic resonance images

Abstract

Purpose

Materials and methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Materials and methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 6/2012

Imaging characteristics of metastatic chordoma

Calcific myonecrosis: a two-patient case series

Maple syrup urine disease: diffusion-weighted MRI findings during acute metabolic encephalopathic crisis

Follow-up CT and MR findings of osteoblastic spinal metastatic lesions after stereotactic radiotherapy

Bilateral accessory inferior turbinates and secondary middle turbinates

Variations in feeding arteries of hepatocellular carcinoma located in the left hepatic lobe