Top

Published in:

Open Access 01-01-2013 | Short Communication

Simple and rapid determination of myristicin in human serum

Authors: Andrzej L. Dawidowicz, Michal P. Dybowski

Published in: Forensic Toxicology | Issue 1/2013

Abstract

Myristicin (5-allyl-1-methoxy-2,3-methylenodioxybenzene) is the main component of nutmeg (Myristica fragrans Houtt.) essential oil. The increasing use of myristicin as a cheap hallucinogenic intoxicant, frequently causing fatal cases of myristicin poisoning, requires new methods for determination of this compound in blood. This report describes the rapid, simple, and useful procedure for myristicin analysis in human serum, involving myristicin–protein complex degradation before chromatographic analysis. The developed method is characterized by a high recovery (above 99 %), a low detection limit (6.0 ng/g) and good repeatability (average RDS of 2.01 %).

Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils—a review. Food Chem Toxicol 46:446–475PubMedCrossRef

Barceloux DG (2009) Nutmeg (Myristica fragrans Houtt.). Dis Mon 55:373–379PubMedCrossRef

Lee BK, Kim JH, Jung JW, Choi JW, Han ES, Lee SH, Ko KH, Ryu JH (2005) Myristicin-induced neurotoxicity in human neuroblastoma SK-N-SH cells. Toxicol Lett 157:49–56PubMedCrossRef

Yun CH, Lee HS, Lee HY, Yim SK, Kim KH, Kim E, Yea SS, Guengerich FP (2003) Roles of human liver cytochrome P450 3A4 and 1A2 enzymes in the oxidation of myristicin. Toxicol Lett 137:143–150PubMedCrossRef

Zheng GQ, Kenney PM, Lam LKT (1992) Myristicin: a potential cancer chemopreventive agent from parsley leaf oil. J Agric Food Chem 40:107–110CrossRef

Gupta KP, van Golen KL, Putman KL, Randerath K (1993) Formation and persistence of safrole-DNA adducts over a 10,000-fold dose range in mouse liver. Carcinogenesis 14:1517–1521PubMedCrossRef

Truitt Jr EB (1979) The pharmacology of myristicin and nutmeg. In: Efron DH, Holmstedt B, Kline NS (eds) Ethnopharmacologic search for psychoactive drugs. Raven press, New york, pp 215–222

Bethesda MD (1997) RTECS (registry of toxic effects of chemical substances). Record No.: 13815

Demetriades AK, Wallman PD, McGuiness A, Gavalas MC (2005) Low cost, high risk: accidental nutmeg intoxication. Emerg Med J 22:223–225PubMedCrossRef

10.

Sangalli BC, Chiang W (2000) Toxicology of nutmeg abuse. Clin Toxicol 38:671–678CrossRef

11.

Forrester MB (2005) Nutmeg intoxication in Texas, 1998–2004. Hum Exp Toxicol 24:563–566PubMedCrossRef

12.

Stein U, Greyer H, Hentschel H (2001) Nutmeg (myristicin) poisoning—report on a fatal case and a series of cases recorded by a poison information centre. Forensic Sci Int 118:87–90PubMedCrossRef

13.

Weil AT (1996) The use of nutmeg as a psychotropic agent. Bull Narc 4:15–23

14.

Lee HS, Jeong TC, Kim JH (1998) In vitro and in vivo metabolism of myristicin in the rat. J Chromatogr B 705:367–372CrossRef

15.

Lee HS, Kim JH, Kim K, Do KS (1998) Determination of myristicin in rat serum samples using microbore high performance liquid chromatography with column-switching. Chromatographia 48:365–368CrossRef

16.

Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectrometry, 4th edn. Allured Publishing Corporation, Carol Stream

17.

Center for Drug Evaluation and Research (CDER), US Food and Drug Administration (FDA) (1996) Guide for Industry. Q2B Validation of Analytical Methods Protocol. Metrology. http://www.fda.gov./CDER/guidance/index.htm

18.

Center for Drug Evaluation and Research (CDER), US Food and Drug Administration (FDA) (1994) Reviewer Guidance. Validation of Chromatographic Methods. www.fda.gov/CDER

19.

Dawidowicz AL, Dybowski MP (2010) SPE isolation of low-molecular oxygen compounds from essential oils. J Sep Sci 33:3213–3220PubMedCrossRef

Title: Simple and rapid determination of myristicin in human serum
Authors: Andrzej L. Dawidowicz
Michal P. Dybowski
Publication date: 01-01-2013
Publisher: Springer Japan
Published in: Forensic Toxicology / Issue 1/2013
Print ISSN: 1860-8965
Electronic ISSN: 1860-8973
DOI: https://doi.org/10.1007/s11419-012-0151-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Simple and rapid determination of myristicin in human serum

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 1/2013

New cannabimimetic indazole derivatives, N-(1-amino-3-methyl-1-oxobutan-2-yl)-1-pentyl-1H-indazole-3-carboxamide (AB-PINACA) and N-(1-amino-3-methyl-1-oxobutan-2-yl)-1-(4-fluorobenzyl)-1H-indazole-3-carboxamide (AB-FUBINACA) identified as designer drugs in illegal products

A case of drowning lacking typical autopsy findings of carbon monoxide poisoning despite the high CO concentration

Identification of (1-pentylindol-3-yl)-(2,2,3,3-tetramethylcyclopropyl)methanone and its 5-pentyl fluorinated analog in herbal incense seized for drug trafficking

Identification of N-ethyl-α-ethylphenethylamine in crystalline powder seized for suspected drug trafficking: a research chemical or a new designer drug?

Cross-reactivities of 41 new amphetamine designer drugs to EMIT® immunoassays

Identification and quantitation of JWH-213, a cannabimimetic indole, as a designer drug in a herbal product