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01-01-2018 | Original Paper

Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554^T in the presence of animal cells

Authors: Yasumasa Hara, Midori A. Arai, Kanae Sakai, Naoki Ishikawa, Tohru Gonoi, Takashi Yaguchi, Masami Ishibashi

Published in: Journal of Natural Medicines | Issue 1/2018

Abstract

A new amide, named dehydropropylpantothenamide (1), was obtained by a co-culture of Nocardia tenerifensis IFM 10554^T in the presence of the mouse macrophage-like cell line J774.1 in modified Czapek-Dox (mCD) medium. Compound 1 was synthesized from d-pantothenic acid calcium salt in 6 steps. The absolute configuration of natural compound 1 was determined by comparisons of the optical rotation and CD spectra of synthetic 1. In the present study, a new method for producing secondary metabolites was demonstrated using a “co-culture” in which the genus Nocardia was cultured in the presence of an animal cell line.

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Title: Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of animal cells
Authors: Yasumasa Hara
Midori A. Arai
Kanae Sakai
Naoki Ishikawa
Tohru Gonoi
Takashi Yaguchi
Masami Ishibashi
Publication date: 01-01-2018
Publisher: Springer Singapore
Published in: Journal of Natural Medicines / Issue 1/2018
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-017-1161-y

At a glance: The STEP trials

Springer Medicine

Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554^T in the presence of animal cells

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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