Top

Published in:

01-01-2017 | Original Paper

Gastroprotective effect of palmatine against acetic acid-induced gastric ulcers in rats

Authors: Ling Wang, Xue Wang, Su-Li Zhang, Xiao-Ming Zhu, Yan-Qi Liu, Zi-Jing Song, Wen-Juan Du, Jun Ji, Cheng-Long Cui, Xin He, Chun-Feng Zhang, Chang-Run Guo, Chong-Zhi Wang, Chun-Su Yuan

Published in: Journal of Natural Medicines | Issue 1/2017

Abstract

Gastric ulcers are one of the most common gastrointestinal disorders. The aim of this study was to investigate the gastroprotective activity and possible underlying mechanisms of palmatine against acetic acid-induced gastric ulcers in rats. Palmatine was administered orally for 7 consecutive days to treat ulcers. The ulcer area, ulcer inhibition rate, histological section, platelet-activating factor (PAF) level in serum, prostaglandin E₂ (PGE₂) level in gastric tissue, 5-hydroxytryptamine (5-HT) level in the brain and norepinephrine (NE) level in the adrenal glands were analyzed. Histological results showed that the ulcer areas were significantly decreased by both doses of palmatine (10 and 20 mg/kg/day) compared with the model group, and the ulcer inhibition rates were 51.42% and 60.92%, respectively. Palmatine treatment markedly increased the level of PGE₂ and decreased PAF, compared with the model group; however, it had no significant effect on 5-HT and NE levels. The results indicated that palmatine may exert a gastroprotective effect against gastric ulcers, and the mechanisms might be associated with the anti-inflammatory status and the protection of gastric mucosa via increasing PGE₂ and decreasing PAF rather than neurohumoral regulation through 5-HT and NE. Thus, palmatine is a potential drug for treatment of gastric ulcers.

Srikanta B, Siddaraju M, Dharmesh S (2007) A novel phenol-bound pectic polysaccharide from Decalepis hamiltonii with multi-step ulcer preventive activity. World J Gastroenterol 13:5196–5207CrossRefPubMedPubMedCentral

Laloo D, Prasad SK, Krishnamurthy S, Hemalatha S (2013) Gastroprotective activity of ethanolic root extract of Potentilla fulgens Wall. ex Hook. J Ethnopharmacol 146:505–514CrossRefPubMed

Saxena B, Singh S (2011) Investigations on gastroprotective effect of citalopram, an antidepressant drug against stress and pyloric ligation induced ulcers. Pharmacol Rep 63:1413–1426CrossRefPubMed

Antonisamy P, Arasu MV, Dhanasekaran M, Choi KC, Aravinthan A, Kim NS, Kang CW, Kim JH (2015) Protective effects of trigonelline against indomethacin-induced gastric ulcer in rats and potential underlying mechanisms. Food Funct 7:398–408CrossRef

Bi WP, Man HB, Man MQ (2014) Efficacy and safety of herbal medicines in treating gastric ulcer: a review. World J Gastroenterol 20:17020–17028CrossRefPubMedPubMedCentral

Yandrapu H, Sarosiek J (2015) Protective factors of the gastric and duodenal mucosa: an overview. Curr Gastroenterol Rep 17:24CrossRefPubMed

Prescott SM, Zimmerman GA, Stafforini DM, McIntyre TM (2000) Platelet-activating factor and related lipid mediators. Annu Rev Biochem 69:419–445CrossRefPubMed

Konturek SJ, Konturek PC, Brzozowski T (2005) Prostaglandins and ulcer healing. J Physiol Pharmacol 56(Suppl 5):5–31

Levy B, Perez P, Perny J, Thivilier C, Gerard A (2011) Comparison of norepinephrine-dobutamine to epinephrine for hemodynamics, lactate metabolism, and organ function variables in cardiogenic shock. A prospective, randomized pilot study. Crit Care Med 39:450–455CrossRefPubMed

10.

DeVault KR, Talley NJ (2009) Insights into the future of gastric acid suppression. Nat Rev Gastroenterol Hepatol 6:524–532CrossRefPubMed

11.

Ji CX, Fan DS, Li W, Guo L, Liang ZL, Xu RM, Zhang JJ (2012) Evaluation of the anti-ulcerogenic activity of the antidepressants duloxetine, amitriptyline, fluoxetine and mirtazapine in different models of experimental gastric ulcer in rats. Eur J Pharmacol 691:46–51CrossRefPubMed

12.

Li ZC, Kong XB, Mai WP, Sun GC, Zhao SZ (2015) Synthesis and antimicrobial activity of 9-o-substituted palmatine derivatives. Indian J Pharm Sci 77:196–201CrossRefPubMedPubMedCentral

13.

Xian X, Sun B, Ye X, Zhang G, Hou P, Gao H (2014) Identification and analysis of alkaloids in cortex Phellodendron amurense by high-performance liquid chromatography with electrospray ionization mass spectrometry coupled with photodiode array detection. J Sep Sci 37:1533–1545CrossRefPubMed

14.

Chao J, Lu TC, Liao JW, Huang TH, Lee MS, Cheng HY, Ho LK, Kuo CL, Peng WH (2009) Analgesic and anti-inflammatory activities of ethanol root extract of Mahonia oiwakensis in mice. J Ethnopharmacol 125:297–303CrossRefPubMed

15.

Xiao CW, Ji QA, Wei Q, Liu Y, Bao GL (2015) Antifungal activity of berberine hydrochloride and palmatine hydrochloride against Microsporum canis-induced dermatitis in rabbits and underlying mechanism. BMC Complement Altern Med 15:177CrossRefPubMedPubMedCentral

16.

Ning N, He K, Wang Y, Zou Z, Wu H, Li X, Ye X (2015) Hypolipidemic effect and mechanism of palmatine from Coptis chinensis in hamsters fed high-fat diet. Phytother Res 29:668–673CrossRefPubMed

17.

Zhu M, Han F, Chen H, Peng Z, Chen Y (2007) Identification of palmatine and its metabolites in rat urine by liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom 21:2019–2022CrossRefPubMed

18.

Jung J, Choi JS, Jeong CS (2014) Inhibitory activities of palmatine from Coptis chinensis against Helicobactor pylori and gastric damage. Toxicol Res 30:45–48CrossRefPubMedPubMedCentral

19.

Okabe S, Pfeiffer CJ (1972) Chronicity of acetic acid ulcer in the rat stomach. Am J Dig Dis 17:619–629CrossRefPubMed

20.

Asante M, Ahmed H, Patel P, Davis T, Finlayson C, Mendall M, Northfield T (1997) Gastric mucosal hydrophobicity in duodenal ulceration: role of Helicobacter pylori infection density and mucus lipids. Gastroenterology 113:449–454CrossRefPubMed

21.

Guerrero AT, Zaperlon AC, Vieira SM, Pinto LG, Ferreira SH, Cunha FQ Jr, Verri WA, Cunha TM (2013) The role of PAF/PAFR signaling in zymosan-induced articular inflammatory hyperalgesia. Naunyn Schmiedebergs Arch Pharmacol 386:51–59CrossRefPubMed

22.

Hermoso M, Barrera N, Morales B, Pérez S, Villalón M (2001) Platelet activating factor increases ciliary activity in the hamster oviduct through epithelial production of prostaglandin E2. Pflugers Arch 442:336–345CrossRefPubMed

23.

Koga MM, Bizzarro B, Sá-Nunes A, Rios FJO, Jancar S (2013) Activation of PAF-receptor induces regulatory dendritic cells through PGE₂ and IL-10. Prostaglandins Leukot Essent Fatty Acids 89:319–326CrossRefPubMed

24.

Castro Faria Neto HC, Stafforini DM, Prescott SM, Zimmerman GA (2005) Regulating inflammation through the anti-inflammatory enzyme platelet-activating factor-acetylhydrolase. Mem Inst Oswaldo Cruz 100(Suppl 1):83–91CrossRefPubMed

25.

Yost CC, Weyrich AS, Zimmerman GA (2010) The platelet activating factor (PAF) signaling cascade in systemic inflammatory responses. Biochimie 92:692–697CrossRefPubMedPubMedCentral

26.

Jimenez MP, Sainz SR (1997) The role of prostaglandins in the defense of the gastric mucosal barrier. Gastroenterol Hepatol 20(Suppl 2):16–21

27.

Rieke C, Kahne T, Schweitzer K, Schraven B, Wienands J, Engelke M, Naumann M (2010) Non-T cell activation linker regulates ERK activation in Helicobacter pylori-infected epithelial cells. Cell Signal 22:395–403CrossRefPubMed

28.

Kim YM, Ha YM, Jin YC, Shi LY, Lee YS, Kim HJ, Seo HG, Choi JS, Kim YS, Kang SS, Lee JH, Chang KC (2009) Palmatine from Coptidis rhizoma reduces ischemia-reperfusion-mediated acute myocardial injury in the rat. Food Chem Toxicol 47:2097–2102CrossRefPubMed

29.

Kraehenmann R, Preller KH, Scheidegger M, Pokorny T, Bosch OG, Seifritz E, Vollenweider FX (2015) Psilocybin-induced decrease in amygdala reactivity correlates with enhanced positive mood in healthy volunteers. Biol Psychiatry 78:572–581CrossRefPubMed

30.

Feldman S, Weidenfeld J (1998) The excitatory effects of the amygdala on hypothalamo-pituitary-adrenocortical responses are mediated by hypothalamic norepinephrine, serotonin, and CRF-41. Brain Res Bull 45:389–393CrossRefPubMed

31.

Zhang S, Xu Z, Gao Y, Wu Y, Li Z, Liu H, Zhang C (2012) Bidirectional crosstalk between stress-induced gastric ulcer and depression under chronic stress. PLoS One 7:e51148CrossRefPubMedPubMedCentral

32.

Malison RT, Price LH, Berman R, van Dyck CH, Pelton GH, Carpenter L, Sanacora G, Owens MJ, Nemeroff CB, Rajeevan N, Baldwin RM, Seibyl JP, Innis RB, Charney DS (1998) Reduced brain serotonin transporter availability in major depression as measured by [123I]-2 beta-carbomethoxy-3 beta-(4-iodophenyl)tropane and single photon emission computed tomography. Biol Psychiatry 44:1090–1098CrossRefPubMed

33.

Meyer JH (2007) Imaging the serotonin transporter during major depressive disorder and antidepressant treatment. J Psychiatry Neurosci 32:86–102PubMedPubMedCentral

34.

Sanchez C, Bergqvist PB, Brennum LT, Gupta S, Hogg S, Larsen A, Wiborg O (2003) Escitalopram, the S-(+)-enantiomer of citalopram, is a selective serotonin reuptake inhibitor with potent effects in animal models predictive of antidepressant and anxiolytic activities. Psychopharmacology 167:353–362CrossRefPubMed

35.

Owens MJ, Knight DL, Nemeroff CB (2001) Second-generation SSRIs: human monoamine transporter binding profile of escitalopram and R-fluoxetine. Biol Psychiatry 50:345–350CrossRefPubMed

Title: Gastroprotective effect of palmatine against acetic acid-induced gastric ulcers in rats
Authors: Ling Wang
Xue Wang
Su-Li Zhang
Xiao-Ming Zhu
Yan-Qi Liu
Zi-Jing Song
Wen-Juan Du
Jun Ji
Cheng-Long Cui
Xin He
Chun-Feng Zhang
Chang-Run Guo
Chong-Zhi Wang
Chun-Su Yuan
Publication date: 01-01-2017
Publisher: Springer Japan
Published in: Journal of Natural Medicines / Issue 1/2017
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-016-1057-2

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Gastroprotective effect of palmatine against acetic acid-induced gastric ulcers in rats

Abstract

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 1/2017

5′-AMP-activated protein kinase plays an essential role in geniposide-regulated glucose-stimulated insulin secretion in rat pancreatic INS-1 β cells

Screening of promising chemotherapeutic candidates from plants against human adult T-cell leukemia/lymphoma (V): coumarins and alkaloids from Boenninghausenia japonica and Ruta graveolens

Treatment of adult and pediatric high-grade gliomas with Withaferin A: antitumor mechanisms and future perspectives

The action and mechanism of myrislignan on A549 cells in vitro and in vivo

Cannabidiolic acid-mediated selective down-regulation of c-fos in highly aggressive breast cancer MDA-MB-231 cells: possible involvement of its down-regulation in the abrogation of aggressiveness

De novo transcriptome assembly and characterization of nine tissues of Lonicera japonica to identify potential candidate genes involved in chlorogenic acid, luteolosides, and secoiridoid biosynthesis pathways