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01-04-2016 | Natural Resource Letter

Anti-miroestrol polyclonal antibodies: a comparison of immunogen preparations used to obtain desired antibody properties

Authors: Tharita Kitisripanya, Kamonthip Jutathis, Chadathorn Inyai, Jukrapun Komaikul, Orapin Udomsin, Gorawit Yusakul, Hiroyuki Tanaka, Waraporn Putalun

Published in: Journal of Natural Medicines | Issue 2/2016

Abstract

Immunogen quality is one important factor that contributes to desirable antibody characteristics. Highly specific antibodies against miroestrol can be used to develop a quality control immunoassay for Pueraria candollei products. In this study, we investigated how various immunogen preparations affect antibody properties. The results show that immunogen prepared using the Mannich reaction provides antibodies with higher specificity and sensitivity against miroestrol than immunogen prepared with the periodate reaction. The results suggest the Mannich reaction maintains the original structure of miroestrol and generates useful antibodies for developing immunoassays.

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Title: Anti-miroestrol polyclonal antibodies: a comparison of immunogen preparations used to obtain desired antibody properties
Authors: Tharita Kitisripanya
Kamonthip Jutathis
Chadathorn Inyai
Jukrapun Komaikul
Orapin Udomsin
Gorawit Yusakul
Hiroyuki Tanaka
Waraporn Putalun
Publication date: 01-04-2016
Publisher: Springer Japan
Published in: Journal of Natural Medicines / Issue 2/2016
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-015-0949-x

At a glance: The STEP trials

Springer Medicine

Anti-miroestrol polyclonal antibodies: a comparison of immunogen preparations used to obtain desired antibody properties

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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