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Journal of Natural Medicines
Published in: Journal of Natural Medicines 2/2012

01-04-2012 | Note

Hypoglycemic effects of clove (Syzygium aromaticum flower buds) on genetically diabetic KK-Ay mice and identification of the active ingredients

Authors: Minpei Kuroda, Yoshihiro Mimaki, Takayuki Ohtomo, Junji Yamada, Tozo Nishiyama, Tatsumasa Mae, Hideyuki Kishida, Teruo Kawada

Published in: Journal of Natural Medicines | Issue 2/2012

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Abstract

Clove (Syzygium aromaticum flower buds) EtOH extract significantly suppressed an increase in blood glucose level in type 2 diabetic KK-Ay mice. In-vitro evaluation showed the extract had human peroxisome proliferator-activated receptor (PPAR)-γ ligand-binding activity in a GAL4-PPAR-γ chimera assay. Bioassay-guided fractionation of the EtOH extract resulted in the isolation of eight compounds, of which dehydrodieugenol (2) and dehydrodieugenol B (3) had potent PPAR-γ ligand-binding activities, whereas oleanolic acid (4), a major constituent in the EtOH extract, had moderate activity. Furthermore, 2 and 3 were shown to stimulate 3T3-L1 preadipocyte differentiation through PPAR-γ activation. These results indicate that clove has potential as a functional food ingredient for the prevention of type 2 diabetes and that 2–4 mainly contribute to its hypoglycemic effects via PPAR-γ activation.
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Metadata
Title
Hypoglycemic effects of clove (Syzygium aromaticum flower buds) on genetically diabetic KK-Ay mice and identification of the active ingredients
Authors
Minpei Kuroda
Yoshihiro Mimaki
Takayuki Ohtomo
Junji Yamada
Tozo Nishiyama
Tatsumasa Mae
Hideyuki Kishida
Teruo Kawada
Publication date
01-04-2012
Publisher
Springer Japan
Published in
Journal of Natural Medicines / Issue 2/2012
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI
https://doi.org/10.1007/s11418-011-0593-z

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