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01-06-2021 | Sleep Apnea | Sleep Breathing Physiology and Disorders • Original Article

Intermittent hypoxia induces turbinate mucosal hypertrophy via upregulating the gene expression related to inflammation and EMT in rats

Authors: Yo-ichiro Kuma, Jun Hosomichi, Hideyuki Maeda, Shuji Oishi, Risa Usumi-Fujita, Yasuhiro Shimizu, Sawa Kaneko, Jun-ichi Suzuki, Ken-ichi Yoshida, Takashi Ono

Published in: Sleep and Breathing | Issue 2/2021

Abstract

Purpose

Chronic intermittent hypoxia (IH) plays a pivotal role in the consequences of obstructive sleep apnea (OSA). It has been demonstrated that IH impairs nasomaxillary complex growth to reduce nasal airway cavity size in rodent models. Although turbinate dysfunction with inflammatory mucosal hypertrophy is related to OSA, the role of IH in turbinate hypertrophy with inflammation-driven fibrosis is unknown. Here, we aimed to clarify the pathogenesis of inflammatory mucosal hypertrophy and epithelial-mesenchymal transition (EMT) in the nasal turbinate under IH.

Methods

Seven-week-old male Sprague-Dawley rats were exposed to IH (4% O₂ to 21% O₂ with 0% CO₂) at a rate of 20 cycles/h.

Results

Hypertrophy of the turbinate mucosa occurred after 3 weeks, with the turbinate mucosa of the experimental group becoming significantly thicker than in the control group. Immunostaining showed that IH increased the expression of TGFβ and N-cadherin and decreased E-cadherin expression in the turbinate mucosa. Quantitative PCR analysis demonstrated that IH enhanced the expression of not only the inflammatory markers Tnf-a, Il-1b, and Nos2 but also the EMT markers Tgf-b1, Col1a1, and Postn.

Conclusions

Collectively, these results suggest that IH induced turbinate hypertrophy via upregulation of gene expression related to inflammation and EMT in the nasal mucosa.

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Title: Intermittent hypoxia induces turbinate mucosal hypertrophy via upregulating the gene expression related to inflammation and EMT in rats
Authors: Yo-ichiro Kuma
Jun Hosomichi
Hideyuki Maeda
Shuji Oishi
Risa Usumi-Fujita
Yasuhiro Shimizu
Sawa Kaneko
Jun-ichi Suzuki
Ken-ichi Yoshida
Takashi Ono
Publication date: 01-06-2021
Publisher: Springer International Publishing
Keyword: Sleep Apnea
Published in: Sleep and Breathing / Issue 2/2021
Print ISSN: 1520-9512
Electronic ISSN: 1522-1709
DOI: https://doi.org/10.1007/s11325-020-02162-6

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Intermittent hypoxia induces turbinate mucosal hypertrophy via upregulating the gene expression related to inflammation and EMT in rats

Abstract

Purpose

Methods

Results

Conclusions

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