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01-03-2021 | Sleep Apnea | Sleep Breathing Physiology and Disorders • Original Article

The dorsal and the ventral side of hypoglossal motor nucleus showed different response to chronic intermittent hypoxia in rats

Authors: Rui Cao, Min-Juan Zhang, Yun-Tao Zhou, Ya-Jie Liu, Huan-Huan Wang, Qin-Xin Zhang, Ya-Wen Shi, Jia-Chen Li, Thian-Sze Wong, Min Yin

Published in: Sleep and Breathing | Issue 1/2021

Abstract

Purpose

To study neurochemical reactions to chronic intermittent hypoxia (CIH) in the hypoglossal nucleus (HN) of rats.

Methods

Adult male Sprague-Dawley rats (n = 12) were randomly divided into two groups (the CIH and the control group). The CIH rats were housed in a hypoxic chamber with the fraction of oxygen volume alternating between 21% and 5% by providing air for 60 s and then providing nitrogen for 60 s from 8:30 am to 16:30 pm each day for 35 days. The control group was housed in a cabin with normal oxygen levels. We studied the expression of c-fos protein, 5-hydroxytryptamine (5-HT) positive terminals, and its 2A receptors in hypoglossal nuclei by immunohistochemistry.

Results

The expression of c-fos, 5-HT positive terminals, and accordingly 5-HT 2A receptors in the CIH group were significantly higher than that in the controls (p < 0.05). The ventral side of the HN showed a clearly higher expression of 5-HT and its 2A receptors than the dorsal side (p < 0.05).

Conclusion

There were 2 responses of the HN to CIH. First, CIH induced a higher expression of 5-HT positive terminals and its 2A receptors, and second, this reaction was much more evident in ventral side than in the dorsal side. We postulate that these responses may serve to be a protective and compensatory mechanism for CIH.

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Title: The dorsal and the ventral side of hypoglossal motor nucleus showed different response to chronic intermittent hypoxia in rats
Authors: Rui Cao
Min-Juan Zhang
Yun-Tao Zhou
Ya-Jie Liu
Huan-Huan Wang
Qin-Xin Zhang
Ya-Wen Shi
Jia-Chen Li
Thian-Sze Wong
Min Yin
Publication date: 01-03-2021
Publisher: Springer International Publishing
Keyword: Sleep Apnea
Published in: Sleep and Breathing / Issue 1/2021
Print ISSN: 1520-9512
Electronic ISSN: 1522-1709
DOI: https://doi.org/10.1007/s11325-020-02125-x

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The dorsal and the ventral side of hypoglossal motor nucleus showed different response to chronic intermittent hypoxia in rats

Abstract

Purpose

Methods

Results

Conclusion

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