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Open Access 01-10-2016 | Research Article

Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET: Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [¹¹C]MPDX

Authors: Jürgen W. A. Sijbesma, Xiaoyun Zhou, David Vállez García, Martin C. Houwertjes, Janine Doorduin, Chantal Kwizera, Bram Maas, Peter Meerlo, Rudi A. Dierckx, Riemer H. J. A. Slart, Philip H. Elsinga, Aren van Waarde

Published in: Molecular Imaging and Biology | Issue 5/2016

Abstract

Purpose

Small animal positron emission tomography (PET) can be used to detect small changes in neuroreceptor availability. This often requires rapid arterial blood sampling. However, current catheterization procedures do not allow repeated blood sampling. We have developed a procedure which allows arterial sampling on repeated occasions in the same animal.

Procedures

Eleven male Wistar rats were two times catheterized via a superficial branch of a femoral artery and scanned with [¹¹C]MPDX and blood sampling. PET images were co-registered to a magnetic resonance imaging (MRI) template. Regional tracer distribution volumes (V _T) in the brain were calculated by the Logan analysis. The procedure was repeated after 1 week.

Results

Surgery was successful in 90 % of the cases, and discomfort was minor. The V _T data showed small differences between test and retest, low between subject variability, and a strong agreement between and within subjects.

Conclusion

Repeated quantitative imaging with a high reproducibility is possible with this approach.

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Title: Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET: Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [11C]MPDX
Authors: Jürgen W. A. Sijbesma
Xiaoyun Zhou
David Vállez García
Martin C. Houwertjes
Janine Doorduin
Chantal Kwizera
Bram Maas
Peter Meerlo
Rudi A. Dierckx
Riemer H. J. A. Slart
Philip H. Elsinga
Aren van Waarde
Publication date: 01-10-2016
Publisher: Springer US
Published in: Molecular Imaging and Biology / Issue 5/2016
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-016-0954-9

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Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET: Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [¹¹C]MPDX

Abstract

Purpose

Procedures

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusion

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