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Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 4/2015

01-08-2015 | Research Article

Assessing Amide Proton Transfer (APT) MRI Contrast Origins in 9 L Gliosarcoma in the Rat Brain Using Proteomic Analysis

Authors: Kun Yan, Zongming Fu, Chen Yang, Kai Zhang, Shanshan Jiang, Dong-Hoon Lee, Hye-Young Heo, Yi Zhang, Robert N. Cole, Jennifer E. Van Eyk, Jinyuan Zhou

Published in: Molecular Imaging and Biology | Issue 4/2015

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Abstract

Purpose

To investigate the biochemical origin of the amide photon transfer (APT)-weighted hyperintensity in brain tumors.

Procedures

Seven 9 L gliosarcoma-bearing rats were imaged at 4.7 T. Tumor and normal brain tissue samples of equal volumes were prepared with a coronal rat brain matrix and a tissue biopsy punch. The total tissue protein and the cytosolic subproteome were extracted from both samples. Protein samples were analyzed using two-dimensional gel electrophoresis, and the proteins with significant abundance changes were identified by mass spectrometry.

Results

There was a significant increase in the cytosolic protein concentration in the tumor, compared to normal brain regions, but the total protein concentrations were comparable. The protein profiles of the tumor and normal brain tissue differed significantly. Six cytosolic proteins, four endoplasmic reticulum proteins, and five secreted proteins were considerably upregulated in the tumor.

Conclusions

Our experiments confirmed an increase in the cytosolic protein concentration in tumors and identified several key proteins that may cause APT-weighted hyperintensity.
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Metadata
Title
Assessing Amide Proton Transfer (APT) MRI Contrast Origins in 9 L Gliosarcoma in the Rat Brain Using Proteomic Analysis
Authors
Kun Yan
Zongming Fu
Chen Yang
Kai Zhang
Shanshan Jiang
Dong-Hoon Lee
Hye-Young Heo
Yi Zhang
Robert N. Cole
Jennifer E. Van Eyk
Jinyuan Zhou
Publication date
01-08-2015
Publisher
Springer US
Published in
Molecular Imaging and Biology / Issue 4/2015
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-015-0828-6

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