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Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 2/2014

01-04-2014 | Brief Article

Monitoring Dynamic Interactions Between Breast Cancer Cells and Human Bone Tissue in a Co-culture Model

Authors: Christopher H. Contag, Wen-Rong Lie, Marie C. Bammer, Jonathan W. Hardy, Tobi L. Schmidt, William J. Maloney, Bonnie L. King

Published in: Molecular Imaging and Biology | Issue 2/2014

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Abstract

Purpose

Bone is a preferential site of breast cancer metastasis, and models are needed to study this process at the level of the microenvironment. We have used bioluminescence imaging (BLI) and multiplex biomarker immunoassays to monitor dynamic breast cancer cell behaviors in co-culture with human bone tissue.

Procedures

Femur tissue fragments harvested from hip replacement surgeries were co-cultured with luciferase-positive MDA-MB-231-fLuc cells. BLI was performed to quantify breast cell proliferation and track migration relative to bone tissue. Breast cell colonization of bone tissues was assessed with immunohistochemistry. Biomarkers in co-culture supernatants were profiled with MILLIPLEX® immunoassays.

Results

BLI demonstrated increased MDA-MB-231-fLuc cell proliferation (p < 0.001) in the presence vs. absence of bones and revealed breast cell migration toward bone. Immunohistochemistry illustrated MDA-MB-231-fLuc cell colonization of bone, and MILLIPLEX® profiles of culture supernatants suggested breast/bone crosstalk.

Conclusions

Breast cell behaviors that facilitate metastasis occur reproducibly in human bone tissue co-cultures and can be monitored and quantified using BLI and multiplex immunoassays.
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Metadata
Title
Monitoring Dynamic Interactions Between Breast Cancer Cells and Human Bone Tissue in a Co-culture Model
Authors
Christopher H. Contag
Wen-Rong Lie
Marie C. Bammer
Jonathan W. Hardy
Tobi L. Schmidt
William J. Maloney
Bonnie L. King
Publication date
01-04-2014
Publisher
Springer US
Published in
Molecular Imaging and Biology / Issue 2/2014
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-013-0685-0

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