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01-04-2014 | Research Article

Comparison of PET Imaging with ⁶⁴Cu-Liposomes and ¹⁸F-FDG in the 7,12-Dimethylbenz[a]anthracene (DMBA)-Induced Hamster Buccal Pouch Model of Oral Dysplasia and Squamous Cell Carcinoma

Authors: Lisa M. Mahakian, D. Gregory Farwell, Hua Zhang, Jai Woong Seo, Brian Poirier, Steven P. Tinling, Alaa M. Afify, Eric M. Haynam, David Shaye, Katherine W. Ferrara

Published in: Molecular Imaging and Biology | Issue 2/2014

Abstract

Purpose

Currently, 2-deoxy-2-[¹⁸F]fluoro-d-glucose (¹⁸F-FDG) is the gold standard radiotracer for staging of head and neck cancer; however, the low sensitivity of this tracer can impede detection of early lesions. ⁶⁴Cu-liposomes accumulate in various cancers and provide both a sensitive tracer and an indication of the biodistribution of nanotherapeutics. Here, the accumulation of ⁶⁴Cu-liposomes in early and established cancers is assessed and compared with ¹⁸F-FDG in a head and neck cancer model.

Methods

Lesions ranging from mild dysplasia to squamous cell carcinoma were induced in a hamster model of head and neck cancer by topical application of 7,12-dimethylbenz[a]anthracene to the buccal pouch. The hamsters were imaged with micro-positron emission tomography using ¹⁸F-FDG and ⁶⁴Cu-liposomes.

Results

At 24 h postinjection, ⁶⁴Cu-liposome accumulation exceeded the accumulation of ¹⁸F-FDG in every pathologic grade. The lesion-to-cheek pouch (background) ratio and lesion-to-brain ratio were also higher for ⁶⁴Cu-liposomes than for ¹⁸F-FDG.

Conclusion

Imaging of a nanotracer such as ⁶⁴Cu-liposomes can improve the visualization of head and neck tumors. Accumulation of liposomal particles in head and neck tumors over various pathologic grades averaged 3.5 %ID/cc demonstrating the potential for liposomal therapy with targeted chemotherapeutic agents.

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Title: Comparison of PET Imaging with 64Cu-Liposomes and 18F-FDG in the 7,12-Dimethylbenz[a]anthracene (DMBA)-Induced Hamster Buccal Pouch Model of Oral Dysplasia and Squamous Cell Carcinoma
Authors: Lisa M. Mahakian
D. Gregory Farwell
Hua Zhang
Jai Woong Seo
Brian Poirier
Steven P. Tinling
Alaa M. Afify
Eric M. Haynam
David Shaye
Katherine W. Ferrara
Publication date: 01-04-2014
Publisher: Springer US
Published in: Molecular Imaging and Biology / Issue 2/2014
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-013-0676-1

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Comparison of PET Imaging with ⁶⁴Cu-Liposomes and ¹⁸F-FDG in the 7,12-Dimethylbenz[a]anthracene (DMBA)-Induced Hamster Buccal Pouch Model of Oral Dysplasia and Squamous Cell Carcinoma

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

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Abstract

Purpose

Methods

Results

Conclusion

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