In Vivo Molecular Imaging of Apoptosisand Necrosis in Atherosclerotic PlaquesUsing MicroSPECT-CT and MicroPET-CT Imaging | springermedicine.com

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Molecular Imaging and Biology

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01-04-2014 | Research Article

In Vivo Molecular Imaging of Apoptosisand Necrosis in Atherosclerotic PlaquesUsing MicroSPECT-CT and MicroPET-CT Imaging

Authors: M. De Saint-Hubert, M. Bauwens, N. Deckers, M. Drummen, K. Douma, P. Granton, G. Hendrikx, D. Kusters, J. Bucerius, C. P. M. Reutelingsperger, F. M. Mottaghy

Published in: Molecular Imaging and Biology | Issue 2/2014

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Abstract

Purpose

The purpose of this paper is to study molecular imaging of apoptosis and necrosis, two key players in atherosclerosis instability, using a multimodal imaging approach combining single photon emission computed tomography (SPECT), positron emission tomography (PET), and computed tomography (CT).

Procedures

Collar-induced carotid atherosclerosis ApoE knockout mice were imaged with ^99mTc-AnxAF568 SPECT-CT to study apoptosis and sequentially with PET-CT following ¹²⁴I-Hypericin (¹²⁴I-Hyp) injection to visualize necrosis.

Results

SPECT depicted increased ^99mTc-AnxAF568 uptake in both atherosclerotic carotid arteries, whereas our data suggest that this uptake is not merely apoptosis related. Although PET of ¹²⁴I-Hyp was hampered by the slow blood clearance in atherosclerotic mice, ¹²⁴I-Hyp was able to target necrosis in the atherosclerotic plaque.

Conclusion

Both ^99mTc-AnxAF568 and ¹²⁴I-Hyp uptake are increased in atherosclerotic carotid vasculature compared to control arteries. While apoptosis imaging remains challenging, necrosis imaging can be feasible after improving the biodistribution characteristics of the probe.

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Title: In Vivo Molecular Imaging of Apoptosisand Necrosis in Atherosclerotic PlaquesUsing MicroSPECT-CT and MicroPET-CT Imaging
Authors: M. De Saint-Hubert
M. Bauwens
N. Deckers
M. Drummen
K. Douma
P. Granton
G. Hendrikx
D. Kusters
J. Bucerius
C. P. M. Reutelingsperger
F. M. Mottaghy
Publication date: 01-04-2014
Publisher: Springer US
Published in: Molecular Imaging and Biology / Issue 2/2014
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-013-0677-0

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