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Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 5/2013

01-10-2013 | Research Article

Voxel-Based Analysis of Amyloid-Burden Measured with [11C]PiB PET in a Double Transgenic Mouse Model of Alzheimer’s Disease

Authors: Boris von Reutern, Barbara Grünecker, Behrooz H. Yousefi, Gjermund Henriksen, Michael Czisch, Alexander Drzezga

Published in: Molecular Imaging and Biology | Issue 5/2013

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Abstract

Purpose

The purpose of this study is to validate the feasibility of a voxel-based analysis of in vivo amyloid-β positron emission tomography (PET) imaging studies in transgenic mouse models of Alzheimer’s disease.

Procedures

We performed [11C]PiB PET imaging in 20 APP/PS1 mice and 16 age-matched controls, and histologically determined the individual amyloid-β plaque load. Using SPM software, we performed a voxel-based group comparison plus a regression analysis between PiB retention and actual plaque load, both thresholded at p FWE < 0.05. In addition, we carried out an individual ROI analysis in every animal.

Results

The automated voxel-based group comparison allowed us to identify voxels with significantly increased PiB retention in the cortical and hippocampal regions in transgenic animals compared to controls. The voxel-based regression analysis revealed a significant association between this signal increase and the actual cerebral plaque load. The validity of these results was corroborated by the individual ROI-based analysis.

Conclusions

Voxel-based analysis of in vivo amyloid-β PET imaging studies in mouse models of Alzheimer’s disease is feasible and allows studying the PiB retention patterns in whole brain maps. Furthermore, the selected approach in our study also allowed us to establish a quantitative relation between tracer retention and actual plaque pathology in the brain in a voxel-wise manner.
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Metadata
Title
Voxel-Based Analysis of Amyloid-Burden Measured with [11C]PiB PET in a Double Transgenic Mouse Model of Alzheimer’s Disease
Authors
Boris von Reutern
Barbara Grünecker
Behrooz H. Yousefi
Gjermund Henriksen
Michael Czisch
Alexander Drzezga
Publication date
01-10-2013
Publisher
Springer US
Published in
Molecular Imaging and Biology / Issue 5/2013
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-013-0625-z

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