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01-10-2013 | Research Article

Tumor Uptake of Hollow Gold Nanospheres After Intravenous and Intra-arterial Injection: PET/CT Study in a Rabbit VX2 Liver Cancer Model

Authors: Mei Tian, Wei Lu, Rui Zhang, Chiyi Xiong, Joe Ensor, Javier Nazario, James Jackson, Colette Shaw, Katherine A. Dixon, Jennifer Miller, Kenneth Wright, Chun Li, Sanjay Gupta

Published in: Molecular Imaging and Biology | Issue 5/2013

Abstract

Purpose

This study was designed to investigate the intratumoral uptake of hollow gold nanospheres (HAuNS) after hepatic intra-arterial (IA) and intravenous (IV) injection in a liver tumor model.

Materials and methods

Fifteen VX2 tumor-bearing rabbits were randomized into five groups (n = 3 in each group) that received either IV ⁶⁴Cu-labeled PEG-HAuNS (IV-PEG-HAuNS), IA ⁶⁴Cu-labeled PEG-HAuNS (IA-PEG-HAuNS), IV cyclic peptide (RGD)-conjugated ⁶⁴Cu-labeled PEG-HAuNS (IV-RGD-PEG-HAuNS), IA RGD-conjugated ⁶⁴Cu-labeled PEG-HAuNS (IA-RGD-PEG-HAuNS), or IA ⁶⁴Cu-labeled PEG-HAuNS with lipiodol (IA-PEG-HAuNS-lipiodol). The animals underwent PET/CT 1 h after injection, and uptake expressed as percentage of injected dose per gram of tissue (%ID/g) was measured in tumor and major organs. The animals were euthanized 24 h after injection, and tissues were evaluated for radioactivity.

Results

At 1 h after injection, animals in the IA-PEG-HAuNS-lipiodol group showed significantly higher tumor uptake (P < 0.001) and higher ratios of tumor-to-normal liver uptake (P < 0.001) than those in all other groups. The biodistribution of radioactivity 24 h after injection showed that IA delivery of PEG-HAuNS with lipiodol resulted in the highest tumor uptake (0.33 %ID/g; P < 0.001) and tumor-to-normal liver ratio (P < 0.001) among all delivery methods. At 24 h, the IA-RGD-PEG-HAuNS group showed higher tumor uptake than the IA-PEG-HAuNS group (0.20 vs. 0.099 %ID/g; P < 0.001).

Conclusion

Adding iodized oil to IA-PEG-HAuNS maximizes nanoparticle delivery to hepatic tumors and therefore may be useful in targeted chemotherapy and photoablative therapy. PET/CT can be used to noninvasively monitor the biodistribution of radiolabeled HAuNS after IV or IA injection.

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Title: Tumor Uptake of Hollow Gold Nanospheres After Intravenous and Intra-arterial Injection: PET/CT Study in a Rabbit VX2 Liver Cancer Model
Authors: Mei Tian
Wei Lu
Rui Zhang
Chiyi Xiong
Joe Ensor
Javier Nazario
James Jackson
Colette Shaw
Katherine A. Dixon
Jennifer Miller
Kenneth Wright
Chun Li
Sanjay Gupta
Publication date: 01-10-2013
Publisher: Springer US
Published in: Molecular Imaging and Biology / Issue 5/2013
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-013-0635-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Tumor Uptake of Hollow Gold Nanospheres After Intravenous and Intra-arterial Injection: PET/CT Study in a Rabbit VX2 Liver Cancer Model

Abstract

Purpose

Materials and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Materials and methods

Results

Conclusion

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