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Molecular Imaging and Biology

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01-03-2009 | Research Article

Time-Course of Contrast Enhancement in Spleen and Liver with Exia 160, Fenestra LC, and VC

Authors: Inneke Willekens, Tony Lahoutte, Nico Buls, Christian Vanhove, Rudi Deklerck, Axel Bossuyt, Johan de Mey

Published in: Molecular Imaging and Biology | Issue 2/2009

Abstract

Objective

The purpose of this study was to compare the time-course of contrast-enhancement in spleen and liver using Exia 160 in comparison with Fenestra LC and VC in healthy mice.

Procedures

Healthy C57bl/6 mice were used in this study. Fenestra LC and VC was administered intravenously at a dose of 0.1 ml/20 g or 0.2 ml/20 g. Exia 160 at a dose of 0.05 ml/20 g or 0.1 ml/20 g. Each animal underwent a micro-CT scan before contrast injection (baseline) and immediately after contrast injection. Additional scans were performed at 1, 2, 3, 4, 24, and 48 h after contrast administration. The mice who received Exia 160 were also scanned after 15, 30, and 45 min.

Results

The peak enhancement of Exia 160 occurred after 15 min for the spleen and after 30 min for the liver.

Conclusions

Exia 160 allows rapid spleen and liver enhancement. The high iodine content results in small injection volumes.

Wolbarst AB, Hendee WR (2006) Evolving and experimental technologies in medical imaging. Radiology 238:16–39PubMedCrossRef

Badea C, Hedlund LW, Johnson GA (2004) Micro-CT with respiratory and cardiac gating. Med Phys 31(12):3324–3329PubMedCrossRef

Deroose CM, De A, Loening AM, Chow PL et al (2007) Multimodality imaging of tumor xenografts and metastases in mice with combined small-animal PET, small-animal CT, and bioluminescence imaging. J Nucl Med 48:295–303PubMed

De Clerck NM, Meurrens K, Weiler H et al (2004) High-resolution X-ray microtomography for the detection of lung tumors in living mice. Neoplasia 6(4):374–379PubMedCrossRef

Paulus MJ, Gleason SS, Kennel SJ et al (2000) High resolution X-ray computed tomography: an emerging tool for small animal cancer research. Neoplasia 2(1–2):62–70PubMedCrossRef

Lewis JS, Achilefu S, Garbow JR et al (2002) Small animal imaging: current technology and perspectives for oncological imaging. Eur J Cancer 38:2173–2188PubMedCrossRef

Weissleder R, Mahmood U (2001) Molecular imaging. Radiology 219:316–333PubMed

Hu J, Haworth ST, Molthen RC, Dawson CA (2004) Dynamic small animal lung imaging via a postacquisition respiratory gating technique using micro-cone beam computed tomography. Acad Radiol 11:961–970PubMedCrossRef

Stenström M, Olander B, Carlsson CA et al (1998) The use of microtomography to monitor morphological changes in small animals. Appl Radiat Isot 49(5/6):565–570PubMedCrossRef

10.

Cavanaugh D, Johnson E, Price RE et al (2004) In vivo respiratory-gated micro-CT imaging in small-animal oncology models. Mol Imaging 3(1):55–62PubMedCrossRef

11.

Holdsworth DW, Thornton MM (2002) Micro-CT in small animal and specimen imaging. Trends Biotechnol 20(8):34–39CrossRef

12.

Massoud TF, Gambhir SS (2003) Molecular imaging in living subjects: seeing fundamental biological processes in a new light. Genes Devl 17(5):545–580CrossRef

13.

Ford NL, Graham KC, Groom AC et al (2006) Time-course characterization of the computed tomography contrast enhancement of an iodinated blood-pool contrast agent in mice using a volumetric flat-panel equipped computed tomography scanner. Invest Radiol 41:384–390PubMedCrossRef

14.

Kao C-Y, Hoffman EA, Beck KC et al (2003) Long-residence-time nano-scale liposomal iohexol for X-ray-based blood pool imaging. Acad Radiol 10:475–483PubMedCrossRef

15.

Mukundan S, Ghaghada KB, Badea CT et al (2006) A liposomal nanoscale contrast agent for preclinical CT in mice. AJR 186:300–307PubMedCrossRef

16.

Weichert JP, Longino MA, Bakan DA et al (1995) Polyiodinated triglyceride analogs as potential computed tomography imaging agents for the liver. J Med Chem 38:636–646PubMedCrossRef

17.

Weichert JP, Lee FT Jr, Chosy SG et al (2000) Combined hepato-selective and blood-pool contrast agents for the CT detection of experimental liver tumors in rabbits. Radiology 216:865–871PubMed

18.

Bakan DA, Weichert JP, Longino MA et al (2000) Polyiodinated triglyceride lipid emulsions for use as hepatoselective contrast agents in CT: effects of physiochemical properties on biodistribution and imaging profiles. Invest Radiol 35:158–169PubMedCrossRef

19.

Weber SM, Peterson KA, Durkee B et al (2004) Imaging of murine liver tumor using micro-CT with a hepatocyte-selective contast agent: accuracy is depentdent on adequate contrast enhancement. J Surg Research 119:41–45CrossRef

20.

Weichert JP, Lee FT Jr, Longino MA et al (1998) Lipid-based blood-pool CT imaging of the liver. Acad Radiol 5(Suppl 1):S16–S19PubMedCrossRef

21.

Bakan DA, Longino MA, Weichert JP et al (1996) Physicochemical characterization of a synthetic lipid emulsion for hepato-selective delivery of lipophilic compounds: application to polyiodinated triglycerides as contrast agents for computed tomography. J Pharm Sciences 85(9):908–914CrossRef

22.

Loening AM, Gambhir SS (2003) Amide: a free software tool for multimodality medical image analysis. Mol Imaging 2(3):131–137PubMedCrossRef

23.

Desser TS, Rubin DL, Muller H et al (1999) Blood pool and liver enhancement in CT with liposomal iodixanol: comparison with iohexol. Acad Radiol 6:176–183PubMedCrossRef

24.

Torchilin VP, Frank-Kamenetsky MD, Wolf GL (1999) CT visualization of blood pool in rats by using long-circulating, iodine-containing micelles. Acad Radiol 6:61–65PubMedCrossRef

25.

Suckow CE, Stout DB (2008) MicroCT liver contrast agent enhancement over time, dose, and mouse strain. Mol Imaging Biol 10(2):114–120PubMedCrossRef

26.

Ohta S, Lai EW, Morris JC et al (2006) MicroCT for high-resolution imaging of ectopic pheochromocytoma tumors in the liver of nude mice. Int J Cancer 119:2236–2241PubMedCrossRef

27.

Almajdub M, Nejjari M, Poncet G et al (2007) In-vivo high-resolution X-ray microtomography for liver and spleen tumor assessment in mice. Contrast Media Mol Imaging 2:88–93PubMedCrossRef

28.

Walters EB, Panda K, Bankson JA et al (2004) Improved method of in vivo respiratory-gated micro-CT imaging. Phys Med Biol 49:4163–4172PubMedCrossRef

Title: Time-Course of Contrast Enhancement in Spleen and Liver with Exia 160, Fenestra LC, and VC
Authors: Inneke Willekens
Tony Lahoutte
Nico Buls
Christian Vanhove
Rudi Deklerck
Axel Bossuyt
Johan de Mey
Publication date: 01-03-2009
Publisher: Springer-Verlag
Published in: Molecular Imaging and Biology / Issue 2/2009
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-008-0186-8

At a glance: The STEP trials

Springer Medicine

Time-Course of Contrast Enhancement in Spleen and Liver with Exia 160, Fenestra LC, and VC

Abstract

Objective

Procedures

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Procedures

Results

Conclusions

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