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01-03-2009 | Research Article

Imaging and Pharmacokinetics of ⁶⁴Cu-DOTA-HB22.7 Administered by Intravenous, Intraperitoneal, or Subcutaneous Injection to Mice Bearing Non-Hodgkin’s Lymphoma Xenografts

Authors: Shiloh M. Martin, Robert T. O’Donnell, David L. Kukis, Craig K. Abbey, Hayes McKnight, Julie L. Sutcliffe, Joseph M. Tuscano

Published in: Molecular Imaging and Biology | Issue 2/2009

Abstract

Purpose

The aim of the study is to compare the tumor-specific targeting, pharmacokinetics, and biodistribution of ⁶⁴Cu-DOTA-HB22.7 when administered to xenograft-bearing mice intravenously (IV), intraperitoneally (IP), and subcutaneously (SQ).

Procedures

Mice bearing human non-Hodgkin’s lymphoma (NHL) xenografts were injected IV, IP, or SQ with ⁶⁴Cu-DOTA-HB22.7. Xenograft targeting was evaluated by micro positron emission tomography (microPET) and confirmed by organ biodistribution studies. Blood measurements of ⁶⁴Cu were performed to determine the pharmacokinetics and clearance of ⁶⁴Cu-DOTA-HB22.7.

Results

⁶⁴Cu-DOTA-HB22.7 demonstrated equivalent tumor targeting within 24–48 h, regardless of the route of administration. Organ biodistribution confirmed tumor-specific targeting. Blood pharmacokinetics demonstrated that ⁶⁴Cu-DOTA-HB22.7 accessed the bloodstream after IP and SQ administration to a similar degree as IV administration, albeit at a slower rate.

Conclusions

These findings establish ⁶⁴Cu-DOTA-HB22.7 as a potential radioimmunotherapeutic and/or NHL-specific imaging agent. These findings provide evidence that IP and SQ administration can achieve results equivalent to IV administration and may lead to more efficient, reproducible treatment plans for antibody-based therapeutics.

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Title: Imaging and Pharmacokinetics of 64Cu-DOTA-HB22.7 Administered by Intravenous, Intraperitoneal, or Subcutaneous Injection to Mice Bearing Non-Hodgkin’s Lymphoma Xenografts
Authors: Shiloh M. Martin
Robert T. O’Donnell
David L. Kukis
Craig K. Abbey
Hayes McKnight
Julie L. Sutcliffe
Joseph M. Tuscano
Publication date: 01-03-2009
Publisher: Springer-Verlag
Published in: Molecular Imaging and Biology / Issue 2/2009
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-008-0148-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Imaging and Pharmacokinetics of ⁶⁴Cu-DOTA-HB22.7 Administered by Intravenous, Intraperitoneal, or Subcutaneous Injection to Mice Bearing Non-Hodgkin’s Lymphoma Xenografts

Abstract

Purpose

Procedures

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusions

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