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Published in: International Urology and Nephrology 9/2019

01-09-2019 | Urology - Original Paper

Hydrogen sulfide-induced relaxation of the bladder is attenuated in spontaneously hypertensive rats

Authors: Suo Zou, Takahiro Shimizu, Masaki Yamamoto, Shogo Shimizu, Youichirou Higashi, Motoaki Saito

Published in: International Urology and Nephrology | Issue 9/2019

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Abstract

Purpose

To compare hydrogen sulfide (H2S)-induced relaxation on the bladder between normotensive and spontaneously hypertensive rat (SHR), we evaluated the effects of H2S donors (GYY4137 and NaHS) on the micturition reflex and on the contractility of bladder tissues. We also investigated the content of H2S and the expression levels of enzymes related to H2S biosynthesis [cystathionine β-synthase (CBS), 3-mercaptopyruvate sulfurtransferase (MPST), and cysteine aminotransferase (CAT)] in the bladder.

Methods

Eighteen-week-old male normotensive Wistar rats and SHRs were used. Under urethane anesthesia, the effects of intravesically instilled GYY4137 (10−8, 10−7 and 10−6 M) on the micturition reflex were evaluated by cystometry. The effects of NaHS (1 × 10−8–3 × 10−4 M) were evaluated on carbachol (10−5 M)-induced pre-contracted bladder strips. Tissue H2S content was measured by the methylene blue method. The expression levels of these enzymes were investigated by Western blot.

Results

GYY4137 significantly prolonged intercontraction intervals in Wistar rats, but not in SHRs. NaHS-induced relaxation on pre-contracted bladder strips was significantly attenuated in SHRs compared with Wistar rats. The H2S content in the bladder of SHRs was significantly higher than that of Wistar rats. CBS, MPST and CAT were detected in the bladder of Wistar rats and SHRs. The expression levels of MPST in the SHR bladder were significantly higher than those in the Wistar rat bladder.

Conclusion

H2S-induced bladder relaxation in SHRs is impaired, thereby resulting in a compensatory increase of the H2S content in the SHR bladder.
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Metadata
Title
Hydrogen sulfide-induced relaxation of the bladder is attenuated in spontaneously hypertensive rats
Authors
Suo Zou
Takahiro Shimizu
Masaki Yamamoto
Shogo Shimizu
Youichirou Higashi
Motoaki Saito
Publication date
01-09-2019
Publisher
Springer Netherlands
Published in
International Urology and Nephrology / Issue 9/2019
Print ISSN: 0301-1623
Electronic ISSN: 1573-2584
DOI
https://doi.org/10.1007/s11255-019-02222-1

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