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Open Access 01-06-2020 | Glioma | Laboratory Investigation

Polycomb-like 2 regulates PRC2 components to affect proliferation in glioma cells

Authors: Fei Wang, Yongying Gao, Ye Lv, Yanwei Wu, Yongzhen Guo, Fang Du, Shixiong Wang, Jiaxiang Yu, Xiangmei Cao, P. Andy Li

Published in: Journal of Neuro-Oncology | Issue 2/2020

Abstract

Introduction

The Polycomb group (PcG) is an important family of transcriptional regulators that controls growth and tumorigenesis. The PcG mainly consists of two complexes, PRC1 and Polycomb Repressive Complex 2 (PRC2). Polycomb-like 2 (PCL2) is known to interact with the PRC2 protein. The role of PCL2 in the development and progression of glioma is unclear.

Methods

We use The Cancer Genome Atlas (TCGA) database to detect the expression of PCL2 in various tumors. 117 cases of clinical glioma (WHOI–IV) were collected, and PCL2 expression and localization were detected by immunohistochemical staining. Glioma cells U87/U251 were infected with overexpressed and interfered PCL2. CCK8 assay, colony formation assay, EdU method, cell cycle and apoptosis were used to detect cell proliferation and apoptosis. Western blot was used to detect the expression of PRC2-related core proteins. After DZNeP intervention, PRC2 protein expression was again measured to discuss the mechanism of PCL2 action.

Results

TCGA database results and immunohistochemical staining results suggest that PCL2 is highly expressed in gliomas. We found that the PCL2 gene promoted tumor cell proliferation, enhanced the colony formation ability, and increased S phase in the cell cycle. The overexpression of PCL2 upregulated the expression levels of EZH2 and EED (two core members of PRC2), decreased the expression of SUZ12, increased the level of H3K27 trimethylation (H3K27me3), H3K4 dimethylation (H3K4me2), and decreased H3K9 dimethylation (H3K9me2). The result after interfering with PCL2 was the opposite.

Conclusions

As an important accessory protein of PRC2, PCL2 can not only change the expression of PRC2 components, but also affect the expression level of Histone methylation. Therefore, PCL2 may be an important hub for regulating the synergy among PRC2 members. This study revealed PCL2 as a new target for tumor research and open up a new avenue for future research in glioma.

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Title: Polycomb-like 2 regulates PRC2 components to affect proliferation in glioma cells
Authors: Fei Wang
Yongying Gao
Ye Lv
Yanwei Wu
Yongzhen Guo
Fang Du
Shixiong Wang
Jiaxiang Yu
Xiangmei Cao
P. Andy Li
Publication date: 01-06-2020
Publisher: Springer US
Keywords: Glioma
Glioma
Glioma
Published in: Journal of Neuro-Oncology / Issue 2/2020
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-020-03538-0

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Polycomb-like 2 regulates PRC2 components to affect proliferation in glioma cells

Abstract

Introduction

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Introduction

Methods

Results

Conclusions

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