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01-06-2020 | Glioblastoma | Laboratory Investigation

Transferrin receptor 1 targeted optical imaging for identifying glioma margin in mouse models

Authors: Xiang-Rong Ni, Yi-Ying Zhao, Hai-Ping Cai, Zhi-Hui Yu, Jing Wang, Fu-Rong Chen, Yan-Jiao Yu, Guo-Kai Feng, Zhong-Ping Chen

Published in: Journal of Neuro-Oncology | Issue 2/2020

Abstract

Objective

Optical molecular imaging technology that indiscriminately detects intracranial glioblastoma (GBM) can help neurosurgeons effectively remove tumor masses. Transferrin receptor 1 (TfR 1) is a diagnostic and therapeutic target in GBM. A TfR 1-targeted peptide, CRTIGPSVC (CRT), was shown to cross the blood brain barrier (BBB) and accumulate at high levels in GBM tissues. In this study, we synthesized a TfR 1-targeted near-infrared fluorescent (NIRF) probe, Cy5-CRT, for identifying the GBM tissue margin in mouse models.

Methods

We initially confirmed the overexpression of TfR 1 in GBM and the tumor-specific homing ability of Cy5-CRT in subcutaneous and orthotopic GBM mouse models. We then examined the feasibility of Cy5-CRT for identifying the tumor margin in orthotopic GBM xenografts. Finally, we compared Cy5-CRT with the clinically used fluorescein sodium in identifying tumor margins.

Results

Cy5-CRT specifically accumulated in GBM tissues and detected the tumor burden with exceptional contrast in mice with orthotopic GBM, enabling fluorescence-guided GBM resection under NIRF live imaging conditions. Importantly, Cy5-CRT recognized the GBM tissue margin more clearly than fluorescein sodium.

Conclusions

The TfR 1-targeted optical probe Cy5-CRT specifically differentiates tumor tissues from the surrounding normal brain with high sensitivity, indicating its potential application for the precise surgical removal of GBM.

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Title: Transferrin receptor 1 targeted optical imaging for identifying glioma margin in mouse models
Authors: Xiang-Rong Ni
Yi-Ying Zhao
Hai-Ping Cai
Zhi-Hui Yu
Jing Wang
Fu-Rong Chen
Yan-Jiao Yu
Guo-Kai Feng
Zhong-Ping Chen
Publication date: 01-06-2020
Publisher: Springer US
Keywords: Glioblastoma
Glioma
Molecular Imaging
Glioma
Glioblastoma
Glioblastoma
Glioma
Published in: Journal of Neuro-Oncology / Issue 2/2020
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-020-03527-3

At a glance: The STEP trials

Springer Medicine

Transferrin receptor 1 targeted optical imaging for identifying glioma margin in mouse models

Abstract

Objective

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Methods

Results

Conclusions

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