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01-04-2015 | Research Article

Blood–brain barrier permeability change and regulation mechanism after subarachnoid hemorrhage

Authors: Zhiqing Li, Guobiao Liang, Teng Ma, Jingchen Li, Ping Wang, Libo Liu, Bo Yu, Yunhui Liu, Yixue Xue

Published in: Metabolic Brain Disease | Issue 2/2015

Abstract

We aimed to investigate the blood brain barrier (BBB) change caused by subarachnoid hemorrhage (SAH) and to explore the molecular mechanisms of acute brain injury after SAH. The SD rat model of SAH was firstly established by endovascular filament perforation technique. The changes of regional cerebral blood flow (rCBF), BBB permeability and ultrastructure of brain tissue at different time points after SAH were respectively observed by Doppler flowmetry, evans blue extravasation and transmission electron microscopy. Meanwhile, the expression changes of Claudin-5, Occludin, Zo-1 and Caveolin-1 were detected by immunohistochemistry and Western blot. Furthermore, the expressions of Akt, P-Akt and Foxo1A were also measured by Western blot. The change of BBB permeability showed two peaks at 3 and 72 h after SAH, corresponding to the change of rCBF. The BBB tight junction opening can be observed after SAH, and the largest opening was occurred at 3 h and 72 h. There was no significant change in Caveolin-1, Claudin-5 and Akt expressions after SAH (P > 0.05), while Zo-1 and Occludin were significantly down-regulated (P < 0.05). The expression of P-Akt was obviously reduced at 30 min and then increased at 1 and 24 h, while Foxo1A was up-regulated at 1 and 24 h after SAH (P < 0.05). Down-regulated Zo-1 and Occludin, as well as Akt/FOXO signaling pathway may be involved in the regulation of tight junction opening and the BBB permeability in the early stage after SAH.

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Title: Blood–brain barrier permeability change and regulation mechanism after subarachnoid hemorrhage
Authors: Zhiqing Li
Guobiao Liang
Teng Ma
Jingchen Li
Ping Wang
Libo Liu
Bo Yu
Yunhui Liu
Yixue Xue
Publication date: 01-04-2015
Publisher: Springer US
Published in: Metabolic Brain Disease / Issue 2/2015
Print ISSN: 0885-7490
Electronic ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-014-9609-1

ACC 2024 Congress

Springer Medicine

Blood–brain barrier permeability change and regulation mechanism after subarachnoid hemorrhage

Abstract

ACC 2024 Congress

Springer Medicine

Abstract

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