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01-11-2014 | Original Research

Increase in the Expression of CD4 + CD25+ Lymphocytic T Cells in the Indeterminate Clinical Form of Human Chagas Disease After Stimulation With Recombinant Antigens of Trypanosoma cruzi

Authors: Suellen Carvalho de Moura Braz, Adriene Siqueira de Melo, Maria da Glória Aureliano de Melo Cavalcanti, Sílvia Marinho Martins, Wilson de Oliveira Jr, Edimilson Domingos da Silva, Antonio Gomes Pinto Ferreira, Virginia Maria Barros de Lorena, Yara de Miranda Gomes

Published in: Journal of Clinical Immunology | Issue 8/2014

Abstract

Purpose

Regulatory T cells are involved in the clinical course of chronic Chagas disease, possibly because they exercise a control in the patient’s inflammatory response to Trypanosoma cruzi. This study analyzed the levels of CD4 + CD25+ T cells in chronic Chagas disease patients after in vitro stimulation of the peripheral blood mononuclear cells with CRA (Cytoplasmic Repetitive Antigen) or FRA (Flagellar Repetitive Antigen) T. cruzi antigens.

Methods

Groups of patients with the cardiac form and indeterminate form; and non-infected individuals, were selected. The CD4 + CD25+ T lymphocyte population, as well as the FoxP3 expression and the IL10 production, were evaluated by flow cytometry after stimulation with CRA or FRA.

Result

The IND group presented higher levels of CD4 + CD25+ T cells than the CARD group. However, there was no evidence of a relationship between FoxP3 and IL10 with any of the chronic forms.

Conclusions

Our results suggest the possible involvement of CD4 + CD25+ T cells specific to CRA and FRA in controlling the progression of clinical outcomes. Though, further studies are needed to define which mechanisms activate regulatory T cells and lead to pathology control in chronic human Chagas disease.

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Title: Increase in the Expression of CD4 + CD25+ Lymphocytic T Cells in the Indeterminate Clinical Form of Human Chagas Disease After Stimulation With Recombinant Antigens of Trypanosoma cruzi
Authors: Suellen Carvalho de Moura Braz
Adriene Siqueira de Melo
Maria da Glória Aureliano de Melo Cavalcanti
Sílvia Marinho Martins
Wilson de Oliveira Jr
Edimilson Domingos da Silva
Antonio Gomes Pinto Ferreira
Virginia Maria Barros de Lorena
Yara de Miranda Gomes
Publication date: 01-11-2014
Publisher: Springer US
Published in: Journal of Clinical Immunology / Issue 8/2014
Print ISSN: 0271-9142
Electronic ISSN: 1573-2592
DOI: https://doi.org/10.1007/s10875-014-0092-6

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Increase in the Expression of CD4 + CD25+ Lymphocytic T Cells in the Indeterminate Clinical Form of Human Chagas Disease After Stimulation With Recombinant Antigens of Trypanosoma cruzi

Abstract

Purpose

Methods

Result

Conclusions

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