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Journal of Assisted Reproduction and Genetics

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01-04-2020 | Fertility Preservation

The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage

Authors: Pinar Ozcan, Taha Takmaz, Olgu Enis Tok, Sevde Islek, Esra Nur Yigit, Cem Ficicioglu

Published in: Journal of Assisted Reproduction and Genetics | Issue 4/2020

Abstract

Purpose

We evaluated the protective effect of PRP on ovarian function in female rats with cyclophosphamide (Cy)-induced ovarian damage.

Methods

Thirty-two adult female Sprague–Dawley rats were randomly divided into four groups. Group 1 (control-sodium chloride 0.9%; 1 mL/kg, single-dose ip injection), group 2 (Cy); 75 mg/kg, single-dose ip injection and sodium chloride 0.9% (1 mL/kg, single-dose ip injection), group 3 Cy plus PRP, Cy (75 mg/kg, single-dose and PRP (200 μl, single-dose) ip injection), group 4 (PRP, 200 μl, single-dose ip injection). Primordial, antral, and atretic follicle counts; serum anti-Müllerian hormone (AMH) levels; AMH-positive granulosa cells; and gene expression analysis of Ddx4 were assessed.

Results

Serum AMH levels were significantly lower in group 2 compared to groups 1, 3, and 4 (p < 0.01, p < 0.01, and p = 0.04, respectively). A significant difference was found in the primordial, primary, secondary, antral, and atretic follicle counts between all groups (p < 0.01). There was a statistically significant difference in AMH-positive staining primary, secondary, and antral follicles count between the groups (p < 0.01). There was a statistically significant difference in primary, secondary, and antral AMH positive staining follicle intensity score between the groups (p < 0.01). Ddx4 expression in group 4 was highest compared to other groups.

Conclusion

Our study may provide evidence that PRP could protect ovarian function against ovarian damage induced by Cy. It could lead to improved primordial, primary, secondary, and antral follicle numbers.

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Title: The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage
Authors: Pinar Ozcan
Taha Takmaz
Olgu Enis Tok
Sevde Islek
Esra Nur Yigit
Cem Ficicioglu
Publication date: 01-04-2020
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 4/2020
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-020-01689-7

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The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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