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Journal of Assisted Reproduction and Genetics

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01-12-2019 | ICSI | Commentary

Density gradient centrifugation and swim-up for ICSI: useful, unsafe, or just unsuitable?

Authors: Hamilton De Martin, Eduardo P. Miranda, Marcello S. Cocuzza, Pedro A. A. Monteleone

Published in: Journal of Assisted Reproduction and Genetics | Issue 12/2019

Excerpt

Intracytoplasmic sperm injection (ICSI) revolutionized the treatment of male factor infertility [1]. ICSI eliminates all natural selection barriers, since the spermatozoon is directly injected into the oocyte cytoplasm [2]. While visual inspection permits a trained embryologist to identify immature spermatozoa, the damage in sperm DNA itself does not result in any morphological changes, making its identification nearly impossible [3]. Therefore, semen processing prior to ICSI procedures is of utmost importance. …

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Title: Density gradient centrifugation and swim-up for ICSI: useful, unsafe, or just unsuitable?
Authors: Hamilton De Martin
Eduardo P. Miranda
Marcello S. Cocuzza
Pedro A. A. Monteleone
Publication date: 01-12-2019
Publisher: Springer US
Keyword: ICSI
Published in: Journal of Assisted Reproduction and Genetics / Issue 12/2019
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-019-01602-x

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