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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 8/2018

01-08-2018 | Embryo Biology

Assessment of developmental potential of human single pronucleated zygotes derived from conventional in vitro fertilization

Authors: Yoshiteru Kai, Hitomi Moriwaki, Keitaro Yumoto, Kyoko Iwata, Yasuyuki Mio

Published in: Journal of Assisted Reproduction and Genetics | Issue 8/2018

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Abstract

Purpose

The aim of this study was to non-invasively validate the developmental potential of human single pronucleated (1PN) zygotes derived from conventional in vitro fertilization (c-IVF) at the zygote stage.

Methods

Fifty 1PN zygotes derived from 45 patients undergoing c-IVF were used. Immunohistochemistry and fluorescence live cell imaging were used to confirm normal chromosome segregation during the first mitosis. The usefulness of measuring pronuclear diameter was assessed on the basis of the presence or absence of a proper first cleavage and validated by subsequent development.

Results

Although approximately 80% (15/19) of 1PN zygotes contained a diploid genome, immunohistochemistry revealed an unequal distribution of paternal and maternal genomes at the first mitosis. Fluorescence live imaging revealed that 73% (8/11) of 1PN zygotes formed a functional mitotic spindle at the first mitosis resulting from diploid genomes, with 25% (2/8) of these forming a tripolar spindle. 1PN zygotes in which the pronucleus disappeared and that subsequently underwent cleavage had a pronuclear diameter ≥ 32.2 μm. The selection of 1PN zygotes based on pronuclear diameter resulted in zygotes that all formed mitotic spindles with poles during cleavage. Furthermore, 63% (5/8) of these zygotes reached the blastocyst stage.

Conclusions

This study demonstrates the usefulness of a non-invasive assessment of 1PN zygotes derived from c-IVF as an indicator of developmental potential. Furthermore, diploid 1PN zygotes do not always exhibit normal chromosome segregation at the first mitosis. A pronuclear diameter ≥ 32.2 μm just before PN breakdown might be a useful criterion to assess 1PN zygotes that are capable of further development.
Appendix
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Metadata
Title
Assessment of developmental potential of human single pronucleated zygotes derived from conventional in vitro fertilization
Authors
Yoshiteru Kai
Hitomi Moriwaki
Keitaro Yumoto
Kyoko Iwata
Yasuyuki Mio
Publication date
01-08-2018
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 8/2018
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-018-1241-2

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