Top

Journal of Assisted Reproduction and Genetics

Published in:

01-11-2016 | Assisted Reproduction Technologies

Delayed intracytoplasmic sperm injection (ICSI) with trophectoderm biopsy and preimplantation genetic screening (PGS) show increased aneuploidy rates but can lead to live births with single thawed euploid embryo transfer (STEET)

Authors: Nidhee M. Sachdev, James A. Grifo, Frederick Licciardi

Published in: Journal of Assisted Reproduction and Genetics | Issue 11/2016

Abstract

Purpose

The aim of this study was to report the results of IVF with trophectoderm biopsy and preimplantation genetic screening (PGS) following delayed intracytoplasmic sperm injection (ICSI).

Methods

Patients undergoing IVF with PGS and delayed ICSI were included in the study. Indications for delayed ICSI included absent or poor fertilization via standard insemination or more than 50 % immature oocytes, noted post-cumulus stripping for standard ICSI procedure. Delayed ICSI was performed the day after retrieval due to absent or poor fertilization. The immature oocytes were kept in extended culture, and if demonstrated maturity, ICSI was performed. Primary outcome included fertilization rate and blastocyst stage formation, defined by the number of blastocysts for biopsy. Secondary outcome included aneuploidy rate and pregnancy outcomes following single thawed euploid embryo transfers (STEET).

Results

Sixteen patients with delayed ICSI were included in the study. Twelve were due to poor fertilization and four secondary to immature oocytes. A total of 219 oocytes were retrieved; ten were frozen upon patient request, 168 had standard insemination, and 13 had routine ICSI on the day of retrieval. A total of 129 oocytes underwent delayed ICSI. Sixty-three (49 %) fertilized, 19 (14.7 %) reached blastocysts for biopsy; fivw of which were chromosomally normal (26.3 %). Three patients underwent STEET of a delayed ICSI embryo; all three resulted in live births, including one embryo biopsied on day 8 of development.

Conclusion

Fertilization failure or an excessive proportion of immature oocytes in an IVF cycle, necessitating delayed ICSI, showed equivalent fertilization and blast formation rates. With the implementation of trophectoderm biopsy and PGS, these embryos can lead to healthy live born babies.

Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992;340(8810):17–8.CrossRefPubMed

Boldt J, Howe AM, Butler WJ, McDonough PG, Padilla SL. The value of oocyte reinsemination in human in vitro fertilization. Fertil Steril. 1987;48(4):617–23.CrossRefPubMed

Liu DY, Baker HWG. Defective sperm–zona pellucida interaction: a major cause of failure of fertilization in clinical in-vitro fertilization. Hum Reprod. 2000;15(3):702–8. doi:10.1093/humrep/15.3.702.CrossRefPubMed

Palermo GD, Neri QV, Rosenwaks Z. To ICSI or not to ICSI. Semin Reprod Med. 2015;33(02):092–102. doi:10.1055/s-0035-1546825.CrossRef

Beck-Fruchter R, Lavee M, Weiss A, Geslevich Y, Shalev E. Rescue intracytoplasmic sperm injection: a systematic review. Fertil Steril. 2014;101(3):690–8. doi:10.1016/j.fertnstert.2013.12.004.CrossRefPubMed

Johnson JE, Higdon 3rd HL, Boone WR. Effect of human granulosa cell co-culture using standard culture media on the maturation and fertilization potential of immature human oocytes. Fertil Steril. 2008;90(5):1674–9. doi:10.1016/j.fertnstert.2007.06.017.CrossRefPubMed

Nagy ZP, Liu J, Joris H. The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum Reprod. 1995;10(5):1123–9.PubMed

Plachot M, de Grouchy J, Junca AM, Mandelbaum J, Salat-Baroux J, Cohen J. Chromosome analysis of human oocytes and embryos: does delayed fertilization increase chromosome imbalance? Hum Reprod. 1988;3(1):125–7.PubMed

Yuzpe AA, Liu Z, Fluker MR. Rescue intracytoplasmic sperm injection (ICSI)-salvaging in vitro fertilization (IVF) cycles after total or near-total fertilization failure. Fertil Steril. 2000;73(6):1115–9.CrossRefPubMed

10.

Morton PC, Yoder CS, Tucker MJ, Wright G, Brockman WD, Kort HI. Reinsemination by intracytoplasmic sperm injection of 1-day-old oocytes after complete conventional fertilization failure. Fertil Steril. 1997;68(3):488–91.CrossRefPubMed

11.

Strassburger D, Goldstein A, Friedler S, Raziel A, Kasterstein E, Mashevich M, et al. The cytogenetic constitution of embryos derived from immature (metaphase I) oocytes obtained after ovarian hyperstimulation. Fertil Steril. 2010;94(3):971–8. doi:10.1016/j.fertnstert.2009.04.035.CrossRefPubMed

12.

Rubino P, Vigano P, Luddi A, Piomboni P. The ICSI procedure from past to future: a systematic review of the more controversial aspects. Hum Reprod Update. 2015. doi:10.1093/humupd/dmv050.PubMed

13.

Emery BR, Wilcox AL, Aoki VW, Peterson CM, Carrell DT. In vitro oocyte maturation and subsequent delayed fertilization is associated with increased embryo aneuploidy. Fertil Steril. 2005;84(4):1027–9. doi:10.1016/j.fertnstert.2005.04.036.CrossRefPubMed

14.

DeScisciolo C, Wright DL, Mayer JF, Gibbons W, Muasher SJ, Lanzendorf SE. Human embryos derived from in vitro and in vivo matured oocytes: analysis for chromosomal abnormalities and nuclear morphology. J Assist Reprod Genet. 2000;17(5):284–92.CrossRefPubMedPubMedCentral

15.

Coticchio G, Dal-Canto M, Guglielmo MC, Mignini-Renzini M, Fadini R. Human oocyte maturation in vitro. Int J Dev Biol. 2012;56(10-12):909–18. doi:10.1387/ijdb.120135gv.CrossRefPubMed

16.

Dailey T, Dale B, Cohen J, Munne S. Association between nondisjunction and maternal age in meiosis-II human oocytes. Am J Hum Genet. 1996;59(1):176–84.PubMedPubMedCentral

17.

Bernardini L, Borini A, Preti S, Conte N, Flamigni C, Capitanio GL, et al. Study of aneuploidy in normal and abnormal germ cells from semen of fertile and infertile men. Hum Reprod. 1998;13(12):3406–13. doi:10.1093/humrep/13.12.3406.CrossRefPubMed

18.

Van Dyk Q, Lanzendorf S, Kolm P, Hodgen GD, Mahony MC. Incidence of aneuploid spermatozoa from subfertile men: selected with motility versus hemizona-bound. Hum Reprod. 2000;15(7):1529–36.CrossRefPubMed

19.

Tsirigotis M, Nicholson N, Taranissi M, Bennett V, Pelekanos M, Craft I. Late intracytoplasmic sperm injection in unexpected failed fertilization in vitro: diagnostic or therapeutic? Fertil Steril. 1995;63(4):816–9.CrossRefPubMed

20.

Dal Canto M, Brambillasca F, Mignini Renzini M, Coticchio G, Merola M, Lain M, et al. Cumulus cell-oocyte complexes retrieved from antral follicles in IVM cycles: relationship between COCs morphology, gonadotropin priming and clinical outcome. J Assist Reprod Genet. 2012;29(6):513–9. doi:10.1007/s10815-012-9766-2.CrossRefPubMedPubMedCentral

Title: Delayed intracytoplasmic sperm injection (ICSI) with trophectoderm biopsy and preimplantation genetic screening (PGS) show increased aneuploidy rates but can lead to live births with single thawed euploid embryo transfer (STEET)
Authors: Nidhee M. Sachdev
James A. Grifo
Frederick Licciardi
Publication date: 01-11-2016
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 11/2016
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-016-0743-z

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Delayed intracytoplasmic sperm injection (ICSI) with trophectoderm biopsy and preimplantation genetic screening (PGS) show increased aneuploidy rates but can lead to live births with single thawed euploid embryo transfer (STEET)

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 11/2016

What is the contribution of embryo-endometrial asynchrony to implantation failure?

Differential expression pattern of Twist1 in mouse preimplantation embryos suggests its multiple roles during early development

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Maternal obesity in mice not only affects fresh embryo quality but also aggravates injury due to vitrification

Building a model to increase live birth rate through patient-specific optimization of embryo transfer day

Polymorphisms of ICAM-1 and IL-6 genes related to endometriosis in a sample of Brazilian women