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Journal of Assisted Reproduction and Genetics

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01-08-2016 | Gamete Biology

Does combining magnetic-activated cell sorting with density gradient or swim-up improve sperm selection?

Authors: Zeynep Cakar, Burcu Cetinkaya, Duru Aras, Betül Koca, Sinan Ozkavukcu, İskender Kaplanoglu, Alp Can, Ozgur Cinar

Published in: Journal of Assisted Reproduction and Genetics | Issue 8/2016

Abstract

Purpose

The present study aimed to evaluate whether combining the magnetic-activated cell sorting (MACS) with density-gradient (DG) or swim-up (SU) sperm separation techniques can improve sperm selection to obtain higher quality spermatozoa.

Methods

Two commonly used sperm selection techniques, SU and DG, were compared to MACS combined with either SU or DG. Spermatozoa obtained from normozoospermic (n = 10) and oligozoospermic (n = 10) cases were grouped as SU, DG, SU+MACS, and DG+MACS followed by the analysis of sperm morphology, motility, DNA integrity, and the levels of Izumo-1 and PLCZ proteins.

Results

Although spermatozoa obtained by SU or DG when combined with MACS have improved aspects when compared to SU or DG alone, results did not reach a statistically significant level. Moreover, separation with MACS caused a significant loss in the numbers of total and rapid progressive spermatozoa.

Conclusions

Considering the cost/benefit ratio, MACS application together with traditional techniques may only be preferred in certain cases having higher concentrations of spermatozoa, but it does not seem to be an ideal and practical sperm selection technique for routine use.

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Title: Does combining magnetic-activated cell sorting with density gradient or swim-up improve sperm selection?
Authors: Zeynep Cakar
Burcu Cetinkaya
Duru Aras
Betül Koca
Sinan Ozkavukcu
İskender Kaplanoglu
Alp Can
Ozgur Cinar
Publication date: 01-08-2016
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 8/2016
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-016-0742-0

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Does combining magnetic-activated cell sorting with density gradient or swim-up improve sperm selection?

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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