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Journal of Assisted Reproduction and Genetics

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01-03-2014 | Gonadal Physiology and Disease

Altered microRNA and gene expression in the follicular fluid of women with polycystic ovary syndrome

Authors: Lauren W. Roth, Blair McCallie, Ruben Alvero, William B. Schoolcraft, Debra Minjarez, Mandy G. Katz-Jaffe

Published in: Journal of Assisted Reproduction and Genetics | Issue 3/2014

Abstract

Purpose

To determine if microRNAs are differentially expressed in the follicular fluid of women with PCOS compared to fertile oocyte donors and identify associated altered gene expression.

Methods

Women undergoing IVF who met Rotterdam criteria for PCOS or who were fertile oocyte donors were recruited from a private IVF center. Individual follicle fluid was collected at the time of oocyte retrieval. MicroRNA analysis was performed using microarray and validated using real-time PCR on additional samples. Potential gene targets were identified and their expression analyzed by real time PCR.

Results

Microarray profiling of human follicular fluid revealed expression of 235 miRNAs, 29 were differentially expressed between the groups. Using PCR validation, 5 miRNAs (32, 34c, 135a, 18b, and 9) showed significantly increased expression in the PCOS group. Pathway analysis revealed genes involved in insulin regulation and inflammation. Three potential target genes were found to have significantly decreased expression in the PCOS group (interleukin 8, synaptogamin 1, and insulin receptor substrate 2).

Conclusions

MicroRNAs are differentially expressed in the follicular fluid of women with PCOS when compared to fertile oocyte donors. There is also altered expression of potential target genes associated with the PCOS phenotype.

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Title: Altered microRNA and gene expression in the follicular fluid of women with polycystic ovary syndrome
Authors: Lauren W. Roth
Blair McCallie
Ruben Alvero
William B. Schoolcraft
Debra Minjarez
Mandy G. Katz-Jaffe
Publication date: 01-03-2014
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 3/2014
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-0161-4

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Altered microRNA and gene expression in the follicular fluid of women with polycystic ovary syndrome

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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