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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 10/2013

01-10-2013 | Fertility Preservation

Attempted application of bioengineered/biosynthetic supporting matrices with phosphatidylinositol-trisphosphate-enhancing substances to organ culture of human primordial follicles

Authors: Galit Lerer-Serfaty, Nivin Samara, Benjamin Fisch, Michal Shachar, Olga Kossover, Dror Seliktar, Avi Ben-Haroush, Ronit Abir

Published in: Journal of Assisted Reproduction and Genetics | Issue 10/2013

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Abstract

Purpose

To improve human primordial follicle culture.

Methods

Thin or thick ovarian slices were cultured on alginate scaffolds or in PEG-fibrinogen hydrogels with or without bpV (pic), which prevents the conversion of phosphatidylinositol-trisphosphate (PIP3) to phosphatidylinositol-bisphosphate (PIP2) or 740Y-P which converts PIP2 to PIP3. Follicular growth was evaluated by follicular counts, Ki67 immunohistochemistry, and 17β-estradiol (E2) levels.

Results

BpV (pic) had a destructive effect on cultured follicles. Thawed-uncultured samples had more primordial follicles than samples cultured in basic medium and fewer developing follicles than samples cultured in PEG-fibrinogen hydrogels with 740Y-P. There were more atretic follicles in samples cultured on alginate scaffolds than in PEG-fibrinogen hydrogels, and in samples cultured in PEG-fibrinogen hydrogels with 740Y-P than in PEG-fibrinogen hydrogels with basic medium. Ki67 staining was higher in PEG-fibrinogen hydrogels than on alginate scaffolds. E2 levels were higher in thick than in thin slices.

Conclusions

PEG-fibrinogen hydrogels appear to have an advantage over alginate scaffolds for culturing human primordial follicles. Folliculogenesis is not increased in the presence of substances that enhance PIP3 production or with thin rather than thick sectioning.
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Metadata
Title
Attempted application of bioengineered/biosynthetic supporting matrices with phosphatidylinositol-trisphosphate-enhancing substances to organ culture of human primordial follicles
Authors
Galit Lerer-Serfaty
Nivin Samara
Benjamin Fisch
Michal Shachar
Olga Kossover
Dror Seliktar
Avi Ben-Haroush
Ronit Abir
Publication date
01-10-2013
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 10/2013
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-013-0052-8

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