Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 8/2012

01-08-2012 | ASSISTED REPRODUCTION TECHNOLOGIES

The effect of immature oocytes quantity on the rates of oocytes maturity and morphology, fertilization, and embryo development in ICSI cycles

Authors: Iman Halvaei, Mohammad Ali Khalili, Mohammad Hossein Razi, Stefania A. Nottola

Published in: Journal of Assisted Reproduction and Genetics | Issue 8/2012

Login to get access

Abstract

Purpose

The goal was to evaluate the role of the number of retrieved immature oocytes on mature oocyte counts and morphology, and also the rates of fertilization and embryo development in ICSI cycles.

Methods

101 ICSI cycles were included in this prospective evaluation. Patients were divided into 2 groups of A (≤ 2 immature oocytes) and B (> 2 immature oocytes). In sub-analysis, the impacts of the number of GV and MI oocytes were assessed on the rates of fertilization and embryo development. Also, correlations between the numbers of immature and mature oocytes, as well as maternal age between two groups were analyzed. Assessments of oocyte morphology, fertilization, embryo quality and development were done accordingly.

Results

There was no correlation between the immature oocytes quantity with the number of mature ones. There were insignificant differences for embryo development between two groups, but fertilization rate was higher in group A (P = 0.03). In sub-analysis, insignificant differences were observed between two groups of ≤ and >2 GV and MI oocytes for rates of fertilization and embryo development. Also, the rates of clinical pregnancy and delivery were insignificant between groups. The rate of morphologically abnormal oocytes had no significant difference between two groups, except for wide perivitelline space (PVS) which was higher in group A (P = 0.03). There was no significant difference for maternal age between two groups.

Conclusions

In cases with few retrieved immature oocytes, rates of fertilization and incidence of wide PVS may increase, although immature oocytes may not have any negative impacts on early embryo development, or the rates on number of mature oocytes.
Literature
1.
Baerwald AR, Adams GP, Pierson RA. Ovarian antral folliculogenesis during the human menstrual cycle: a review. Hum Reprod Update. 2012;18(1):73–91.PubMedCrossRef
2.
Balaban B, Urman B. Effect of oocyte morphology on embryo development and implantation. Reprod Biomed Online. 2006;12(5):608–15.PubMedCrossRef
3.
Blake M, Garrisi J, Tomkin G, Cohen J. Sperm deposition site during ICSI affects fertilization and development. Fertil Steril. 2000;73(1):31–7.PubMedCrossRef
4.
Child TJ, Sylvestre C, Pirwany I, Tan SL. Basal serum levels of FSH and estradiol in ovulatory and anovulatory women undergoing treatment by in-vitro maturation of immature oocytes. Hum Reprod. 2002;17(8):1997–2002.PubMedCrossRef
5.
de Cassia SFR, de Almeida Ferreira Braga DP, Semiao-Francisco L, Madaschi C, Iaconelli Jr A, Borges Jr E. Metaphase II human oocyte morphology: contributing factors and effects on fertilization potential and embryo developmental ability in ICSI cycles. Fertil Steril. 2010;94(3):1115–7.CrossRef
6.
Durlinger A, Visser JA, Themmen A. Regulation of ovarian function: the role of anti-Mullerian hormone. Reproduction. 2002;124(5):601–9.PubMedCrossRef
7.
Esfandiari N, Javed MH, Gotlieb L, Casper RF. Complete failed fertilization after intracytoplasmic sperm injection–analysis of 10 years' data. Int J Fertil Womens Med. 2005;50(4):187–92.PubMed
8.
Gougeon A. Human ovarian follicular development: from activation of resting follicles to preovulatory maturation. Ann Endocrinol (Paris). 2010;71(3):132–43.CrossRef
9.
Halvaei I, Khalili MA, Soleimani M, Razi MH. Does maternal age have any effect on the rates of fertilization and embryo development in ICSI cycles? Iranian J Reprod Med. 2011;9 Suppl 1:49.
10.
Halvaei I, Khalili MA, Soleimani M, Razi MH. Evaluating the role of first polar body morphology on rates of fertilization and embryo development in ICSI cycles. Int J Fertil Steril. 2011;5(2):110–5.
11.
Hill GA, Freeman M, Bastias MC, Rogers BJ, Herbert CM, Osteen KG, Wentz AC. The influence of oocyte maturity and embryo quality on pregnancy rate in a program for in vitro fertilization-embryo transfer. Fertil Steril. 1989;52(5):801–6.PubMed
12.
Hsu M, Mayer J, Aronshon M, Lanzendorf S, Muasher S, Kolm P, Oehninger S. Embryo implantation in in vitro fertilization and intracytoplasmic sperm injection: impact of cleavage status, morphology grade, and number of embryos transferred. Fertil Steril. 1999;72(4):679–85.PubMedCrossRef
13.
Jayaprakasan K, Deb S, Batcha M, Hopkisson J, Johnson I, Campbell B, Raine-Fenning N. The cohort of antral follicles measuring 2–6 mm reflects the quantitative status of ovarian reserve as assessed by serum levels of anti-Müllerian hormone and response to controlled ovarian stimulation. Fertil Steril. 2010;94(5):1775–81.PubMedCrossRef
14.
Jee BC, Ku SY, Suh CS, Kim KC, Lee WD, Kim SH. Serum anti-Müllerian hormone and inhibin B levels at ovulation triggering day can predict the number of immature oocytes retrieved in in vitro fertilization cycles. J Korean Med Sci. 2008;23(4):657–61.PubMedCrossRef
15.
Khalili MA, Mir-Rokni F, Kalantar SM. Application of vitality tests on asthenozoospermic semen from infertile men. Iran Biomed J. 1999;3:77–81.
16.
Khalili MA, Mojibian M, Sultan AM. Role of oocyte morphology on fertilization and embryo formation in assisted reproductive techniques. Middle East Fertil Soc J. 2005;10(1):72–7.
17.
Kim BK, Lee SC, Kim KJ, Han CH, Kim JH. In vitro maturation, fertilization, and development of human germinal vesicle oocytes collected from stimulated cycles. Fertil Steril. 2000;74(6):1153–8.PubMedCrossRef
18.
Kok JD, Looman CWN, Weima SM, te Velde ER. A high number of oocytes obtained after ovarian hyperstimulation for in vitro fertilization or intracytoplasmic sperm injection is not associated with decreased pregnancy outcome. Fertil Steril. 2006;85(4):918–24.PubMedCrossRef
19.
Lin YH, Hwang JL. In vitro maturation of human oocytes. Taiwan J Obstet Gynecol. 2006;45(2):95–9.PubMedCrossRef
20.
McAvey B, Zapantis A, Jindal SK, Lieman HJ, Polotsky AJ. How many eggs are needed to produce an assisted reproductive technology baby: is more always better? Fertil Steril. 2011;96(2):332–5.PubMedCrossRef
21.
Melie N, Adeniyi O, Igbineweka O, Ajayi R. Predictive value of the number of oocytes retrieved at ultrasound-directed follicular aspiration with regard to fertilization rates and pregnancy outcome in intracytoplasmic sperm injection treatment cycles. Fertil Steril. 2003;80(6):1376–9.PubMedCrossRef
22.
Miao Y, Ma S, Liu X, Miao D, Chang Z, Luo M, Tan J. Fate of the first polar bodies in mouse oocytes. Mol Reprod Dev. 2004;69(1):66–76.PubMedCrossRef
23.
Motta PM, Nottola SA, Micara G, Familiari G. Ultrastructure of human unfertilized oocytes and polyspermic embryos in an IVF–ET program a. Ann N Y Acad Sci. 1988;541(1):367–83.PubMedCrossRef
24.
Nazari S, Khalili MA, Esmaielzadeh F, Mohsenzadeh M. Maturation capacity, morphology and morphometric assessment of human immature oocytes after vitrification and in-vitro maturation. Iranian J Reprod Med. 2011;9(3):209–16.
25.
Palermo GD, Cohen J, Rosenwaks Z. Intracytoplasmic sperm injection: a powerful tool to overcome fertilization failure. Fertil Steril. 1996;65(5):899–908.PubMed
26.
Rienzi L, Ubaldi F, Anniballo R, Cerulo G, Greco E. Preincubation of human oocytes may improve fertilization and embryo quality after intracytoplasmic sperm injection. Hum Reprod. 1998;13(4):1014–9.PubMedCrossRef
27.
Serhal PFRD, Kinis A, Marchant S, Davies M, Khadum IM. Oocyte morphology predicts outcome of intracytoplasmic sperm injection. Hum Reprod. 1997;12:1267–70.PubMedCrossRef
28.
Shen S, Khabani A, Klein N, Battaglia D. Statistical analysis of factors affecting fertilization rates and clinical outcome associated with intracytoplasmic sperm injection. Fertil Steril. 2003;79(2):355–60.PubMedCrossRef
29.
Strassburger D, Friedler S, Raziel A, Schachter M, Kasterstein E, Ron-El R. Very low sperm count affects the result of intracytoplasmic sperm injection. J Assist Reprod Genet. 2000;17(8):431–6.PubMedCrossRef
30.
Svalander P, Forsberg A, Jakobsson A, Wikland M. Factors of importance for the establishment of a successful program of intracytoplasmic sperm injection treatment for male infertility. Fertil Steril. 1995;63(4):828–37.PubMed
31.
Tan SL, Child TJ, Gulekli B. In vitro maturation and fertilization of oocytes from unstimulated ovaries: predicting the number of immature oocytes retrieved by early follicular phase ultrasonography. Am J Obstet Gynecol. 2002;186(4):684–9.PubMedCrossRef
32.
Van Steirteghem AC, Liu J, Joris H, Nagy Z, Janssenswillen C, Tournaye H, Derde MP, Van Assche E, Devroey P. Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles. Hum Reprod. 1993;8(7):1055–60.PubMed
33.
WHO (2010) WHO laboratory manual for the Examination and processing of human semen. 5 edn. Cambridge University Press.
34.
Wittemer C, Ohl J, Bettahar-Lebugle K, Viville S, Nisand I. A quantitative and morphological analysis of oocytes collected during 438 IVF cycles. J Assist Reprod Genet. 2000;17(1):44–50.PubMedCrossRef
35.
Xia P. lntracytoplasmic sperm injection: correlation of oocyte grade based on polar body, perivitelline space and cytoplasmic inclusion with fertilization rate and embryo quality. Hum Reprod. 1997;2:1750–5.CrossRef
36.
Xing X, Zhao H, Li M, Sun M, Li Y, Chen ZJ (2011) Morphologically abnormal oocytes not capable of fertilization despite repeated strategies. Fertility and Sterility 95 (7):2435 e2435-2437
37.
Yoldemir T, Fraser I. The effect of retrieved oocyte count on pregnancy outcomes in an assisted reproduction program. Arch Gynecol Obstet. 2010;281(3):551–6.PubMedCrossRef
Metadata
Title
The effect of immature oocytes quantity on the rates of oocytes maturity and morphology, fertilization, and embryo development in ICSI cycles
Authors
Iman Halvaei
Mohammad Ali Khalili
Mohammad Hossein Razi
Stefania A. Nottola
Publication date
01-08-2012
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 8/2012
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-012-9799-6

Other articles of this Issue 8/2012

Journal of Assisted Reproduction and Genetics 8/2012 Go to the issue

ASSISTED REPRODUCTION TECHNOLOGIES

Random anti-Müllerian hormone predicts ovarian response in women with high baseline follicle-stimulating hormone levels

Gamete Biology

Influence of microsurgical varicocelectomy on human sperm mitochondrial DNA copy number: a pilot study

Fertility Preservation

Embryo transfer following in vitro maturation and cryopreservation of oocytes recovered from antral follicles during conservative surgery for ovarian cancer

Stem Cell Biology

Developmental expression of lineage specific genes in porcine embryos of different origins

Stem Cell Biology

Derivation, culture and retinal pigment epithelial differentiation of human embryonic stem cells using human fibroblast feeder cells

Erratum

Erratum to: Fertilization outcome, embryo development and birth after unstimulated IVM