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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 12/2010

01-12-2010 | Stem Cell Biology

Establishment of mouse embryonic stem cells from isolated blastomeres and whole embryos using three derivation methods

Authors: Sheyla González, Elena Ibáñez, Josep Santaló

Published in: Journal of Assisted Reproduction and Genetics | Issue 12/2010

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Abstract

Purpose

The aim of the present study is to compare three previously described mouse embryonic stem cell derivation methods to evaluate the influence of culture conditions, number of isolated blastomeres and embryonic stage in the derivation process.

Methods

Three embryonic stem cell derivation methods: standard, pre-adhesion and defined culture medium method, were compared in the derivation from isolated blastomeres and whole embryos at 4- and 8-cell stages.

Results

A total of 200 embryonic stem cell lines were obtained with an efficiency ranging from 1.9% to 72%.

Conclusions

Using either isolated blastomeres or whole embryos, the highest rates of mouse embryonic stem cell establishment were achieved with the defined culture medium method and efficiencies increased as development progressed. Using isolated blastomeres, efficiencies increased in parallel to the proportion of the embryo volume used to start the derivation process.
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Metadata
Title
Establishment of mouse embryonic stem cells from isolated blastomeres and whole embryos using three derivation methods
Authors
Sheyla González
Elena Ibáñez
Josep Santaló
Publication date
01-12-2010
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 12/2010
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-010-9473-9

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