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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 12/2010

01-12-2010 | Gamete Biology

Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia

Authors: Bianjiang Liu, Peng Wang, Zengjun Wang, Yuejun Jia, Xiaobing Niu, Wei Wang, Wei Zhang

Published in: Journal of Assisted Reproduction and Genetics | Issue 12/2010

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Abstract

Purpose

To analyze the abundance and difference of voltage-dependent anion channel (VDAC) mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia.

Methods

High motile and low motile spermatozoa were separated respectively from ejaculates of 36 donors and 40 patients using a discontinuous Percoll gradient centrifugation. Real-Time PCR was performed to detect mRNA abundance and difference of three VDAC subtypes between two groups with different sperm motility.

Results

Real-Time PCR demonstrated that three VDAC mRNAs were present in mature spermatozoa. The VDAC2 mRNA level in ejaculated spermatozoa of patients was significantly higher than that of donors. No significant differences of VDAC1 and VDAC3 mRNA levels were found between two groups.

Conclusion

The high abundance of VDAC2 mRNA seemed to have a positive correlation with low sperm motility. The abnormal expression of VDAC might be related to male infertility with idiopathic asthenozoospermia.
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Metadata
Title
Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia
Authors
Bianjiang Liu
Peng Wang
Zengjun Wang
Yuejun Jia
Xiaobing Niu
Wei Wang
Wei Zhang
Publication date
01-12-2010
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 12/2010
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-010-9466-8

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