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Journal of Assisted Reproduction and Genetics

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01-01-2009 | ANIMAL EXPERIMENTATION

Improvement of embryonic development and production of offspring by transferring meiosis-II chromosomes of senescent mouse oocytes into cytoplasts of young mouse oocytes

Authors: Akinori Mitsui, Midori Yoshizawa, Hiromichi Matsumoto, Emiko Fukui

Published in: Journal of Assisted Reproduction and Genetics | Issue 1/2009

Abstract

Purpose

The effects of reciprocal transplantation of meiosis-II chromosomes between senescent and young mouse oocytes were evaluated based on pre- and post-implantation development ability of resultant embryos.

Methods

Karyoplasts including meiosis-II chromosomes of oocytes from senescent Rockefeller mouse/Ms-Rb(6, 15) females (10 to 12 months, age-related infertile mice) were transferred into cytoplasts of oocytes from young F₁ females (3 to 5 months). Reconstructed oocytes were fertilized in vitro, and then the resultant embryos were cultured in vitro and transferred to recipient mice.

Results

The reconstructed oocytes that consisted of aged-karyoplasts and young-cytoplasts showed significantly improved embryonic development (from 23.2% to 30.0%) and development to term (from 6.3% to 27.1%, P < 0.05) as compared with the oocytes reconstructed from young-karyoplasts and aged-cytoplasts.

Conclusions

The present study showed successful rejuvenation for age-related infertility using transplantation of meiosis-II chromosomes in animal experimental models.

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Title: Improvement of embryonic development and production of offspring by transferring meiosis-II chromosomes of senescent mouse oocytes into cytoplasts of young mouse oocytes
Authors: Akinori Mitsui
Midori Yoshizawa
Hiromichi Matsumoto
Emiko Fukui
Publication date: 01-01-2009
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 1/2009
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-008-9282-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Improvement of embryonic development and production of offspring by transferring meiosis-II chromosomes of senescent mouse oocytes into cytoplasts of young mouse oocytes

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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