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Journal of Assisted Reproduction and Genetics

Published in:

01-08-2008 | ASSISTED REPRODUCTION

Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

Authors: Enver Kerem Dirican, Osman Denizhan Özgün, Süleyman Akarsu, Kadir Okhan Akın, Özge Ercan, Mukaddes Uğurlu, Çağrı Çamsarı, Oya Kanyılmaz, Adnan Kaya, Ali Ünsal

Published in: Journal of Assisted Reproduction and Genetics | Issue 8/2008

Abstract

Purpose

Magnetic activated cell sorting (MACS) eliminates apoptotic spermatozoa based on the presence of externalized phosphatidylserine residues. We evaluated the outcome of male fertility treatment when intracytoplasmic sperm injection (ICSI) into human oocytes was performed with non-apoptotic MACS-selected spermatozoa.

Methods

196 couples were treated by ICSI following spermatozoa preparation by MACS (study group; 122 couples) or density gradient centrifugation (DGC) (control group; 74 couples). Fertilization, cleavage, pregnancy, and implantation rates were analyzed.

Results

The percentage of sperm with normal morphology after MACS selection was improved. Cleavage and pregnancy rates were higher, respectively, in the study group than in control. A slightly higher implantation rate was also observed in the study group.

Conclusions

MACS selection of human spermatozoa increased cleavage and pregnancy rates in oligoasthenozoospermic ART cases. This novel method for selecting non-apoptotic spermatozoa for ICSI is safe and reliable, and may improve the assisted reproduction outcome.

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Title: Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction
Authors: Enver Kerem Dirican
Osman Denizhan Özgün
Süleyman Akarsu
Kadir Okhan Akın
Özge Ercan
Mukaddes Uğurlu
Çağrı Çamsarı
Oya Kanyılmaz
Adnan Kaya
Ali Ünsal
Publication date: 01-08-2008
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 8/2008
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-008-9250-1

At a glance: The STEP trials

Springer Medicine

Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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