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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 2-3/2008

01-03-2008 | Animal Experimentation

Evaluation of the developmental competence and chromosomal compliment of mouse oocytes derived from in-vitro growth and maturation of preantral follicles

Authors: Lihua Liu, Vincent W. Aoki, Douglas T. Carrell

Published in: Journal of Assisted Reproduction and Genetics | Issue 2-3/2008

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Abstract

Purpose

To evaluate the developmental potential and aneuploidy rates of in-vitro versus in-vivo grown and matured mouse oocytes.

Methods

Mice were superovulated to obtain in-vivo matured oocytes. Mouse preantral follicles were also mechanically isolated and cultured in-vitro. In-vitro fertilization (IVF) was performed and fertilization, cleavage, and morula/blastocyst formation rates were compared between groups. Cytogenetic analysis was used to compare oocyte aneuploidy rates and aneuploidy characteristics in the developing embryos.

Results

In-vivo oocyte maturation resulted in higher IVF fertilization, cleavage, and morula/blastocyst formation rates versus in-vitro follicle culture (96.4% versus 78.5%, p < 0.001; 95.3% versus 77.4%, p < 0.001; 94.1% versus 76.9%, p < 0.001). Total aneuploidy rates were higher in embryos derived from in-vitro matured oocytes versus those grown in-vivo (4.0% versus 1.3%, p < 0.05).

Conclusions

Results indicate a reduced developmental competency of in-vitro matured oocytes. The data also highlight an increased susceptibility to meiotic errors in early stage follicles undergoing in vitro culture.
Literature
1.
Liu J, Rybouchkin A, Van der Elst J, Dhont M. Fertilization of mouse oocytes from in vitro-matured preantral follicles using classical in vitro fertilization or intracytoplasmic sperm injection. Biol Reprod 2002;67:575–9.PubMedCrossRef
2.
Eppig JJ, O’Brien M, Wigglesworth K. Mammalian oocyte growth and development in vitro. Mol Reprod Dev 1996;44:260–73.PubMedCrossRef
3.
Eppig JJ, Schultz RM, O’Brien M, Chesnel F. Relationship between the developmental programs controlling nuclear and cytoplasmic maturation of mouse oocytes. Dev Biol 1994;164:1–9.PubMedCrossRef
4.
Trounson A, Anderiesz C, Jones G. Maturation of human oocytes in vitro and their developmental competence. Reproduction (Camb) 2001;121:51–75.CrossRef
5.
Rizos D, Ward F, Duffy P, Boland MP, Lonergan P. Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: implications for blastocyst yield and blastocyst quality. Mol Reprod Dev 2002;61:234–48.PubMedCrossRef
6.
Hardy K, Wright CS, Franks S, Winston RM. In vitro maturation of oocytes. Br Med Bull 2000;56:588–602.PubMedCrossRef
7.
Carrell DT, Liu L, Huang I, Peterson CM. Comparison of maturation, meiotic competence, and chromosome aneuploidy of oocytes derived from two protocols for in vitro culture of mouse secondary follicles. J Assist Reprod Genet 2005;22:347–54.PubMedCrossRef
8.
Eppig JJ, O’Brien MJ. Development in vitro of mouse oocytes from primordial follicles. Biol Reprod 1996;54:197–207.PubMedCrossRef
9.
Roy SK, Terada DM. Activities of glucose metabolic enzymes in human preantral follicles: in vitro modulation by follicle-stimulating hormone, luteinizing hormone, epidermal growth factor, insulin-like growth factor I, and transforming growth factor beta1. Biol Reprod 1999;60:763–8.PubMedCrossRef
10.
Roy SK, Treacy BJ. Isolation and long-term culture of human preantral follicles. Fertil Steril 1993;59:783–90.PubMed
11.
12.
Roberts R, Iatropoulou A, Ciantar D, Stark J, Becker DL, Franks S, et al. Follicle-stimulating hormone affects metaphase I chromosome alignment and increases aneuploidy in mouse oocytes matured in vitro. Biol Reprod 2005;72:107–18.PubMedCrossRef
13.
Salamanca F, Armendares S. C bands in human metaphase chromosomes treated by barium hydroxide. Annal Genet 1974;17:135–6.
14.
Adriaens I, Cortvrindt R, Smitz J. Differential FSH exposure in preantral follicle culture has marked effects on folliculogenesis and oocyte developmental competence. Hum Reprod (Oxf) 2004;19:398–408.CrossRef
15.
Hu Y, Betzendahl I, Cortvrindt R, Smitz J, Eichenlaub-Ritter U. Effects of low O2 and ageing on spindles and chromosomes in mouse oocytes from pre-antral follicle culture. Hum Reprod (Oxf) 2001;16:737–48.CrossRef
16.
Sun F, Betzendahl I, Shen Y, Cortvrindt R, Smitz J, Eichenlaub-Ritter U. Preantral follicle culture as a novel in vitro assay in reproductive toxicology testing in mammalian oocytes. Mutagenesis 2004;19:13–25.PubMedCrossRef
17.
Ruangvutilert P, Delhanty JD, Serhal P, Simopoulou M, Rodeck CH, Harper JC. FISH analysis on day 5 post-insemination of human arrested and blastocyst stage embryos. Prenat Diagn 2000;20:552–60.PubMedCrossRef
18.
19.
Lightfoot DA, Kouznetsova A, Mahdy E, Wilbertz J, Hoog C. The fate of mosaic aneuploid embryos during mouse development. Dev Biol 2006;289:384–94.PubMedCrossRef
20.
Everett CA, West JD. The influence of ploidy on the distribution of cells in chimaeric mouse blastocysts. Zygote (Camb) 1996;4:59–66.
21.
Clouston HJ, Herbert M, Fenwick J, Murdoch AP, Wolstenholme J. Cytogenetic analysis of human blastocysts. Prenat Diagn 2002;22:1143–52.PubMedCrossRef
22.
Mackay GE, West JD. Fate of tetraploid cells in 4n<−>2n chimeric mouse blastocysts. Mech Dev 2005;122:1266–81.PubMedCrossRef
23.
Macas E, Rosselli M, Imthurn B, Keller PJ. Chromosomal constitution of mouse blastocysts derived from oocytes inseminated by multiple sperm insertion into the perivitelline space. J Assist Reprod Genet 1993;10:468–75.PubMedCrossRef
24.
Hare WC, Singh EL, Betteridge KJ, Eaglesome MD, Randall GC, Mitchell D, et al. Chromosomal analysis of 159 bovine embryos collected 12 to 18 days after estrus. Can J Genet Cytol 1980;22:615–26.PubMed
25.
Murray JD, Moran C, Boland MP, Nancarrow CD, Sutton R, Hoskinson RM, et al. Polyploid cells in blastocysts and early fetuses from Australian Merino sheep. J Reprod Fertil 1986;78:439–46.PubMedCrossRef
26.
Drury KC, Kovalinskaia L, Williams RS. Polyploidy as a normal function of trophoblast development in human preimplantation embryos observed by fluorescent in situ hybridization (FISH) analysis. Fertil Steril 1998;70:10S.CrossRef
27.
Benkhalifa M, Janny L, Vye P, Malet P, Boucher D, Menezo Y. Assessment of polyploidy in human morulae and blastocysts using co-culture and fluorescent in-situ hybridization. Hum Reprod (Oxf) 1993;8:895–902.
28.
Bongso A, Chye NS, Sathananthan H, Mui-Nee L, Mok H, Wong PC, et al. Chromosome analysis of two-cell mouse embryos frozen by slow and ultrarapid methods using two different cryoprotectants. Fertil Steril 1988;49:908–12.PubMed
29.
Maudlin I, Fraser LR. The effect of PMSG dose on the incidence of chromosomal anomalies in mouse embryos fertilized in vitro. J Reprod Fertil 1977;50:275–80.PubMedCrossRef
30.
Santalo J, Estop AM, Egozcue J. The chromosome complement of first-cleavage mouse embryos after in vitro fertilization. J In Vitro Fert Embryo Transf 1986;3:99–105.PubMedCrossRef
31.
Golubovsky MD. Postzygotic diploidization of triploids as a source of unusual cases of mosaicism, chimerism and twinning. Hum Reprod (Oxf) 2003;18:236–42.CrossRef
32.
Grossmann M, Calafell JM, Brandy N, Vanrell JA, Rubio C, Pellicer A, et al. Origin of tripronucleate zygotes after intracytoplasmic sperm injection. Hum Reprod (Oxf) 1997;12:2762–5.CrossRef
Metadata
Title
Evaluation of the developmental competence and chromosomal compliment of mouse oocytes derived from in-vitro growth and maturation of preantral follicles
Authors
Lihua Liu
Vincent W. Aoki
Douglas T. Carrell
Publication date
01-03-2008
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 2-3/2008
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-008-9201-x

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