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Journal of Assisted Reproduction and Genetics

Published in:

01-03-2008 | Genetics

Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four cells embryos

Authors: I. E. Agerholm, C. Hnida, D. G. Crüger, C. Berg, G. Bruun-Petersen, S. Kølvraa, S. Ziebe

Published in: Journal of Assisted Reproduction and Genetics | Issue 2-3/2008

Abstract

Purpose

The aim was to elucidate if the nuclear size and number are indicative of aberrant chromosome content in human blastomeres and embryos.

Methods

The number of nuclei and the nucleus and blastomere size were measured by a computer controlled system for multilevel analysis. Then the nuclei were enumerated for 13 chromosomes by a combination of PNA and DNA probes.

Results

In the mononucleated embryos there was no difference in the mean size of chromosomally normal and abnormal nuclei but a significant difference in the mean nuclei size of nuclei that had gained chromosomes compared to nuclei that had lost chromosomes. The nuclei from multinucleated blastomeres had a significant smaller mean size and the frequency of chromosomally aberrant blastomeres was significantly higher.

Conclusion

The mean nuclear size is not a marker for the chromosome content in mononucleated embryos. However, it seems that the nuclei size can be related to multinucleation and maybe to the chromosome content.

Ziebe S, Lundin K, Loft A, Bergh C, Nyboe Andersen AN, Selleskog U, et al. FISH analysis for chromosomes 13, 16, 18, 21, 22, X and Y in all blastomeres of IVF pre-embryos from 144 randomly selected donated human oocytes and impact on pre-embryo morphology. Hum Reprod. 2003;18:2575–81.PubMedCrossRef

Kats-Jaffe MG, Trounson AO, Cram DS. Mitotic errors in chromosome 21 of human preimplantation embryos are associated with non-viability. Mol Hum Reprod. 2004;10:143–7.CrossRef

Hardy K, Winston RM, Handyside AH. Binucleate blastomeres in preimplantation human embryos in vitro; failure if cytokinesis during early cleavage. Fertil Steril. 1993;70:60–6.

De Scisciolo C, Wright DL, Mayer JF, Gibbons W, Muasher AJ, Lanzendorf SE. Human embryo derived from in vitro and in vivo matured oocytes: analysis for chromosomal abnormalities and nuclear morphology. J Ass Reprod Gen. 2000;17:284–92.CrossRef

Meriano J, Clark C, Cadesky K, Laskin C. Binucleated and micronucleated blastomeres ind embryos derived from human assisted reproduction cycles. Reprod BioMed Online. 2004;9:511–20.PubMedCrossRef

Kligman I, Bendiva C, Alikani M, Munne S. The presence of multinucleated blastomeres in human embryos is correlated with chromosomal abnormalities. Hum Reprod. 1996;11:1492–8.PubMed

Yakin K. Impact of the presence of one or more multinucleated blastomeres on the developmental potential of the embryo to the blastocyst stage. Fertil Steril. 2005;83:243–5.PubMedCrossRef

Jackson KV, Ginsburg ES, Hornstein MD, Rein MS, Clark RN. Multinucleation in normally fertilized embryos is associated with an accelerated ovulation induction response and lower implantation and pregnancy rates in in vitro fertilization-embryo transfer cycles. Fertil Steril. 1998;70:60–6.PubMedCrossRef

Van Royen E, Mangelschots K, Vercruyssen M, De Neubourg D, Valken burg M, Rychart G, Gerris J. Multinucleation in cleavage stage embryos. Hum Reprod. 2003;18:1062–9.PubMedCrossRef

10.

Moriwaki T, Suganuma N, Hayakawa M, Hibi H, Katsunata Y, Ogushi H, et al. Embryo evaluation by analysing blastomeres nuclei. Hum Reprod. 2004;19:152–6.PubMedCrossRef

11.

Munne S, Cohen J. Unsuitability of multinucleated human blastomeres for preimplantation genetic diagnosis. Hum Reprod. 1993;8:1120–5.PubMed

12.

Hnida C, Engenheiro E, Ziebe S. Computer-controlled, multilevel, morphometric analysis of blastomere size as biomarker of fragmentation and multinuclearity in human embryos. Hum Reprod. 2004;19:288–93.PubMedCrossRef

13.

Hardarson T, Hanson C, Sjogren A, Lundin K. Human embryos with uneven sized blastomeres have lower pregnancy and implantation rates: indication for aneuploidy and multinucleation. Hum Reprod. 2001;16:313–8.PubMedCrossRef

14.

Munne S. Preimplantation genetic diagnosis and human implantation. A review. Placenta 2003;24:1–7.CrossRef

15.

Delhanty JD. Preimplantation genetics: an explanation for poor human fertility? Ann Hum Genet. 2001;65:331–8.PubMedCrossRef

16.

Wells D, Delhanty JD. Comprehensive chromosomal analysis of human preimplantation embryos using whole genome amplification and single cell comparative genomic hybridization. Mol Hum Reprod. 2000;6:1055–62.PubMedCrossRef

17.

Hnida C, Agerholm I, Ziebe S. Traditional detection versus computer-controlled multilevel analysis of nuclear structures from donated human embryos. Hum Reprod. 2005;20:665–71.PubMedCrossRef

18.

Coonen E, Dumoulin CM, Ramaekers FCS, Hopman AHN. Optimal preparation of preimplantation embryo interphase nuclei for analysis by fluorescence in-situ hybridization. Human Reprod. 1994;9:533–7.

19.

Agerholm I, Ziebe S, Williams B, Berg C, Crüger DG, Bruun-Petersen G, et al. Sequential FISH analysis using competitive displacement of labelled peptide nucleic acid probes for eight chromosomes in human blastomeres. Hum Reprod. 2005;20:1072–7.PubMedCrossRef

20.

McKenzie LJ, Carson SA, Marcelli S, Rooney E, Cisneros P, Torsky S, et al. Nuclear chromosomal localization in human preimplantation embryos: correlation with aneuploidy and embryos morphology. Hum Reprod. 2004;19:2231–7.PubMedCrossRef

21.

Pickering SJ, Taylor A, Johnsom MH, Braude PR. An analysis of multinuclear blastomeres and their derivative daughter blastomeres. Hum Reprod. 1995;10:1912–22.PubMed

22.

Delimetreva SM, Zhivkova RS, Vatev ITS, Toncheva DI. Chromosomal disorders and nuclear and cell destruction in cleaving human embryos. Int J Dev Biol. 2005;49:409–16.CrossRef

23.

Saldeen P, Sundstrom P. Nuclear status of for cell preembryos predicts implantation potential in in vitro fertilization treatment cycles. Fertil Steril. 2005;84:584–9.PubMedCrossRef

Title: Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four cells embryos
Authors: I. E. Agerholm
C. Hnida
D. G. Crüger
C. Berg
G. Bruun-Petersen
S. Kølvraa
S. Ziebe
Publication date: 01-03-2008
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 2-3/2008
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-008-9199-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four cells embryos

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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